Recombinant Anti-TPP1 antibody [EPR16537] (ab195234)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-234882-anti-tpp1-antibody-epr16537-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16537] to TPP1
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-TPP1 antibody [EPR16537]
See all TPP1 primary antibodies -
Description
Rabbit monoclonal [EPR16537] to TPP1 -
Host species
Rabbit -
Specificity
This product detects Tripeptidyl-peptidase 1 (TPP1). It is unable to detect Adrenocortical dysplasia protein homolog which is also known as TPP1.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart, HeLa and Jurkat lysates; Human placenta tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16537 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/50 - 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 34, 48, 61 kDa (predicted molecular weight: 34, 61 kDa).
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| Notes |
|---|
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IHC-P
1/50 - 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Detects a band of approximately 34, 48, 61 kDa (predicted molecular weight: 34, 61 kDa). |
Target
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Function
Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus. -
Tissue specificity
Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues. -
Involvement in disease
Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles. -
Sequence similarities
Belongs to the peptidase S53 family. -
Post-translational
modificationsActivated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 1200 Human
- Entrez Gene: 12751 Mouse
- Entrez Gene: 83534 Rat
- Omim: 607998 Human
- SwissProt: O14773 Human
- SwissProt: O89023 Mouse
- SwissProt: Q9EQV6 Rat
- Unigene: 523454 Human
see all -
Alternative names
- Cell growth inhibiting gene 1 protein antibody
- Cell growth-inhibiting gene 1 protein antibody
- Ceroid lipofuscinosis neuronal 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)
Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TPP1 with ab195234 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution and counter-stained with Hematoxylin.
(inset: negative control).Note: Cytoplasm staining on human placenta tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-234884-anti-tpp1-antibody-epr16537-western-blot.jpg)
Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/10000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34, 61 kDa
Exposure time: 3 minutesab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.
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![Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-234881-anti-tpp1-antibody-epr16537-western-blot.jpg)
Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34, 61 kDa
Exposure time: 3 minutesab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.
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![Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-4-benefits-of-recombinant-antibodies.png)
Anti-TPP1 antibody [EPR16537] (ab195234)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab195234 has not yet been referenced specifically in any publications.
