Recombinant Anti-TPP1 antibody [EPR16537] (ab195234)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TPP1 antibody [EPR16537]
See all TPP1 primary antibodies -
Description
Rabbit monoclonal [EPR16537] to TPP1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human TPP1 aa 350-500. The exact sequence is proprietary.
Database link: O14773 -
Positive control
- Human fetal heart, HeLa and Jurkat lysates; Human placenta tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16537 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab195234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 34, 48, 61 kDa (predicted molecular weight: 34, 61 kDa). |
Target
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Function
Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus. -
Tissue specificity
Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues. -
Involvement in disease
Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles. -
Sequence similarities
Belongs to the peptidase S53 family. -
Post-translational
modificationsActivated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 1200 Human
- Entrez Gene: 12751 Mouse
- Entrez Gene: 83534 Rat
- Omim: 607998 Human
- SwissProt: O14773 Human
- SwissProt: O89023 Mouse
- SwissProt: Q9EQV6 Rat
- Unigene: 523454 Human
see all -
Alternative names
- Cell growth inhibiting gene 1 protein antibody
- Cell growth-inhibiting gene 1 protein antibody
- Ceroid lipofuscinosis neuronal 2 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-234882-anti-tpp1-antibody-epr16537-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (ab195234)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TPP1 with ab195234 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution and counter-stained with Hematoxylin.
(inset: negative control).Note: Cytoplasm staining on human placenta tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-234884-anti-tpp1-antibody-epr16537-western-blot.jpg)
Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/10000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34, 61 kDa
Exposure time: 3 minutesab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.
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![Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)](https://www.abcam.com/ps/products/195/ab195234/Images/ab195234-234881-anti-tpp1-antibody-epr16537-western-blot.jpg)
Western blot - Anti-TPP1 antibody [EPR16537] (ab195234)All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 34, 61 kDa
Exposure time: 3 minutesab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.
Datasheets and documents
References
This product has been referenced in:
- Margalef P et al. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell 172:439-453.e14 (2018). Read more (PubMed: 29290468) »
