Overview

  • Product name

    Anti-TPP1 antibody [EPR16537]
    See all TPP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR16537] to TPP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human TPP1 aa 350-500. The exact sequence is proprietary.
    Database link: O14773

  • Positive control

    • Human fetal heart, HeLa and Jurkat lysates; Human placenta tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/150. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 34, 48, 61 kDa (predicted molecular weight: 34, 61 kDa).

Target

  • Function

    Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.
  • Tissue specificity

    Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.
  • Involvement in disease

    Defects in TPP1 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2) [MIM:204500]. A form of neuronal ceroid lipofuscinosis. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN2 consists of curvilinear profiles.
  • Sequence similarities

    Belongs to the peptidase S53 family.
  • Post-translational
    modifications

    Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity.
  • Cellular localization

    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cell growth inhibiting gene 1 protein antibody
    • Cell growth-inhibiting gene 1 protein antibody
    • Ceroid lipofuscinosis neuronal 2 antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile (Jansky Bielschowsky disease) antibody
    • Ceroid lipofuscinosis neuronal 2 late infantile antibody
    • CLN 2 antibody
    • CLN2 antibody
    • GIG 1 antibody
    • GIG1 antibody
    • Growth inhibiting protein 1 antibody
    • LPIC antibody
    • Lysosomal pepstatin insensitive protease antibody
    • Lysosomal pepstatin-insensitive protease antibody
    • MGC21297 antibody
    • TPP 1 antibody
    • TPP I antibody
    • TPP-1 antibody
    • TPP-I antibody
    • Tpp1 antibody
    • TPP1_HUMAN antibody
    • TPPI antibody
    • Tripeptidyl aminopeptidase antibody
    • Tripeptidyl peptidase I antibody
    • Tripeptidyl-peptidase 1 antibody
    • Tripeptidyl-peptidase I antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling TPP1 with ab195234 at 1/150 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution and counter-stained with Hematoxylin.
    (inset: negative control).

    Note: Cytoplasm staining on human placenta tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/10000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34, 61 kDa


    Exposure time: 3 minutes


    ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.

  • All lanes : Anti-TPP1 antibody [EPR16537] (ab195234) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34, 61 kDa


    Exposure time: 3 minutes


    ab195234 recognises both 61kDa pro-form, 48kDa mature form and 34kDa shorter isoform.

References

This product has been referenced in:

  • Margalef P  et al. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell 172:439-453.e14 (2018). Read more (PubMed: 29290468) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
25 µg
Specification
Lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 15 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (lung cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
lung cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 09 2016

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