Product nameAnti-TPPP antibody [EPR3315]
See all TPPP primary antibodies
DescriptionRabbit monoclonal [EPR3315] to TPPP
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Human
Synthetic peptide within Human TPPP aa 1-100. The exact sequence is proprietary.
- Human fetal brain and SH-SY5Y cell lysates; Human astrocytoma tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as Tubulin Polymerization Promoting Protein.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab119987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min.|
|IP||1/10 - 1/100.|
FunctionMay play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the TPPP family.
modificationsPoor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
- Information by UniProt
- 25 kDa brain specific protein antibody
- 25 kDa brain-specific protein antibody
- Brain specific protein p25 alpha antibody
All lanes : Anti-TPPP antibody [EPR3315] (ab119987) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 23 kDa
ab119987 at 1/100 dilution staining TPPP in Paraffin-embedded Human astrocytoma tissue by Immunohistochemistry.
ab119987 has not yet been referenced specifically in any publications.