Anti-TPPP2 antibody (ab121215)
Key features and details
- Rabbit polyclonal to TPPP2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TPPP2 antibody
See all TPPP2 primary antibodies -
Description
Rabbit polyclonal to TPPP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 8-150 of Human TPPP2.
-
Positive control
- Human kidney tissue; TPPP2 transfected HEK293T lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
May bind tubulin but has no microtubule bundling activity. -
Sequence similarities
Belongs to the TPPP family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 122664 Human
- SwissProt: P59282 Human
- Unigene: 406966 Human
-
Alternative names
- C14orf8 antibody
- Gm1790 antibody
- Gm77 antibody
see all
Images
-
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121215 1-4 µg/ml. Cells treated with PFA/Triton X-100.
-
ab121215 at 1/150 dilution staining TPPP2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
-
All lanes : Anti-TPPP2 antibody (ab121215) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over expression Lysate (Coexpressed with a C terminal myc DDK tag (~3.1 kDa) in mammalian HEK293T cells
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab121215 has been referenced in 2 publications.
- Zhu F et al. PRSS55 plays an important role in the structural differentiation and energy metabolism of sperm and is required for male fertility in mice. J Cell Mol Med 25:2040-2051 (2021). PubMed: 33417308
- Zhu F et al. Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice. J Cell Mol Med 23:2583-2594 (2019). PubMed: 30680919