Overview

  • Product name

  • Description

    Rabbit polyclonal to TPPP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 8-150 of Human TPPP2.

  • Positive control

    • Human kidney tissue; TPPP2 transfected HEK293T lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May bind tubulin but has no microtubule bundling activity.
  • Sequence similarities

    Belongs to the TPPP family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • C14orf8 antibody
    • Gm1790 antibody
    • Gm77 antibody
    • P18 antibody
    • p25beta antibody
    • Protein p25-beta antibody
    • RGD1566185 antibody
    • TPPP/p18 antibody
    • TPPP2 antibody
    • TPPP2_HUMAN antibody
    • Tubulin polymerization-promoting protein family member 2 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and cytoplasm. Recommended concentration of ab121215 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121215 at 1/150 dilution staining TPPP2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • All lanes : Anti-TPPP2 antibody (ab121215) at 1/250 dilution

    Lane 1 : Negative control (vector only transfected HEK293T lysate)
    Lane 2 : Over expression Lysate (Coexpressed with a C terminal myc DDK tag (~3.1 kDa) in mammalian HEK293T cells

References

This product has been referenced in:

  • Zhu F  et al. Deficiency of TPPP2, a factor linked to oligoasthenozoospermia, causes subfertility in male mice. J Cell Mol Med 23:2583-2594 (2019). Read more (PubMed: 30680919) »
See 1 Publication for this product

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