Product nameAnti-TPR antibody
See all TPR primary antibodies
DescriptionRabbit polyclonal to TPR
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TPR aa 2300-2349 (C terminal). The exact sequence is proprietary. NP_003283.1
Database link: P12270
- IHC-P: Human testis tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/2000 - 1/10000. Predicted molecular weight: 267 kDa.|
FunctionComponent of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
Tissue specificityHighest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
Involvement in diseaseDefects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
- Information by UniProt
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
All lanes : Anti-TPR antibody (ab264153) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Predicted band size: 267 kDa
Exposure time: 30 seconds
TPR was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264153 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264153 at 1 µg/ml.
Lane 1: ab264153 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 1 sec.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling TPR with ab264153 at 1/100 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264153 has not yet been referenced specifically in any publications.