• Product name

  • Description

    Rabbit polyclonal to TPR
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1650 and 1700 of human TPR

  • Positive control

    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab70610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 300 kDa (predicted molecular weight: 267 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
  • Tissue specificity

    Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
  • Involvement in disease

    Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
    Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Nucleoprotein TPR antibody
    • tpr antibody
    • TPR_HUMAN antibody
    • translocated promoter region (to activated MET oncogene) antibody


  • All lanes : Anti-TPR antibody (ab70610) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 267 kDa
    Observed band size: 300 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 235 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds
  • Detection of Human TPR by Western Blot of Immunprecipitate. ab70610 at 1µg/ml staining TPR in HeLa whole cell lysates immunoprecipitated using ab70610 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 second.


This product has been referenced in:

  • López-Soop G  et al. AKAP95 interacts with nucleoporin TPR in mitosis and is important for the spindle assembly checkpoint. Cell Cycle 16:947-956 (2017). Read more (PubMed: 28379780) »
  • Myers KN  et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . Read more (PubMed: 27739501) »
See all 2 Publications for this product

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