Key features and details
- Rabbit polyclonal to TPR
- Suitable for: IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TPR antibody
See all TPR primary antibodies
DescriptionRabbit polyclonal to TPR
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
Synthetic peptide, corresponding to a region between C terminal amino acids 2300-2349 of Human TPR (NP_003283.1).
- Human Breast Carcinoma, Colon Carcinoma, Hodgkins Lymphoma, Linitis Plastica Stomach Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Skin Squamous Cell Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma and mouse Squamous Cell Carcinoma tissue.
Concentration is optimized for IHC and not determined
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity purified using an epitope specific to TPR immobilized on solid support.
Our Abpromise guarantee covers the use of ab84516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.
Epitope exposure is recommended. Exposure with citrate buffer will enhance staining. Likely to work with frozen sections.
|ICC/IF||1/100 - 1/500.
Permeabilization with Triton-X 100 is recommended.
FunctionComponent of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
Tissue specificityHighest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
Involvement in diseaseDefects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
- Information by UniProt
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
ab84516 at a 1/250 dilution, staining in human TPR in human prostate carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
ab84516 at a 1:250 dilution, staining mouse TPR in mouse squamous cell carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
ab84516 at a 1/250 dilution, staining human TPR in NBF-fixed asynchronous HeLa cells by immunocytochemistry. Detection: anti-rabbit IgG-FITC conjugate used at a dilution of 1/100.
ab84516 at a 1/250 dilution, staining TPR in human breast carcinoma, by immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Detection: DAB staining using anti-Rabbit secondary antibody at a dilution of 1/100.
ab84516 has been referenced in 10 publications.
- Fišerová J et al. Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution. Cell Mol Life Sci 76:2199-2216 (2019). PubMed: 30762072
- McCloskey A et al. Tpr regulates the total number of nuclear pore complexes per cell nucleus. Genes Dev 32:1321-1331 (2018). PubMed: 30228202
- Liu S et al. Nuclear envelope assembly defects link mitotic errors to chromothripsis. Nature 561:551-555 (2018). PubMed: 30232450
- Fišerová J et al. Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data. J Cell Sci 130:2066-2077 (2017). PubMed: 28476938
- Lång A et al. Visualization of PML nuclear import complexes reveals FG-repeat nucleoporins at cargo retrieval sites. Nucleus 8:404-420 (2017). PubMed: 28402725
- Pérez-Garrastachu M et al. Nucleoporins redistribute inside the nucleus after cell cycle arrest induced by histone deacetylases inhibition. Nucleus 8:515-533 (2017). PubMed: 28696859
- Andronov L et al. ClusterViSu, a method for clustering of protein complexes by Voronoi tessellation in super-resolution microscopy. Sci Rep 6:24084 (2016). PubMed: 27068792
- Guo Y & Zheng Y Lamins position the nuclear pores and centrosomes by modulating dynein. Mol Biol Cell 26:3379-89 (2015). ICC/IF . PubMed: 26246603
- Guo Y et al. Concentration-dependent lamin assembly and its roles in the localization of other nuclear proteins. Mol Biol Cell 25:1287-97 (2014). ICC/IF ; Mouse . PubMed: 24523288
- Umlauf D et al. The human TREX-2 complex is stably associated with the nuclear pore basket. J Cell Sci 126:2656-67 (2013). Human . PubMed: 23591820