Key features and details
- Rabbit polyclonal to TPR - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-TPR antibody - C-terminal
See all TPR primary antibodies
DescriptionRabbit polyclonal to TPR - C-terminal
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
Synthetic peptide within Human TPR (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P12270
- ICC/IF: A431 cells. WB: Mouse lung tissue extract.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 267 kDa.|
FunctionComponent of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
Tissue specificityHighest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
Involvement in diseaseDefects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
- Information by UniProt
- Nucleoprotein TPR antibody
- tpr antibody
- TPR_HUMAN antibody
- translocated promoter region (to activated MET oncogene) antibody
A431 (Human epidermoid carcinoma cell line) cells stained for TPR (green) using ab229352 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
Anti-TPR antibody - C-terminal (ab229352) at 1/500 dilution + Mouse lung tissue extract at 30 µg
Developed using the ECL technique.
Predicted band size: 267 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229352 has not yet been referenced specifically in any publications.