Overview

  • Product name

    Anti-TPR antibody - C-terminal
    See all TPR primary antibodies
  • Description

    Rabbit polyclonal to TPR - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide within Human TPR (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: P12270

  • Positive control

    • ICC/IF: A431 cells. WB: Mouse lung tissue extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 267 kDa.

Target

  • Function

    Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
  • Tissue specificity

    Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
  • Involvement in disease

    Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
    Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • Nucleoprotein TPR antibody
    • tpr antibody
    • TPR_HUMAN antibody
    • translocated promoter region (to activated MET oncogene) antibody

Images

  • A431 (Human epidermoid carcinoma cell line) cells stained for TPR (green) using ab229352 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-TPR antibody - C-terminal (ab229352) at 1/500 dilution + Mouse lung tissue extract at 30 µg

    Developed using the ECL technique.

    Predicted band size: 267 kDa



    5% SDS-PAGE

References

ab229352 has not yet been referenced specifically in any publications.

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