RecombinantRabMAb

Recombinant Anti-TPR antibody [EPR8982] (ab170940)

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Western blot - Anti-TPR antibody [EPR8982] (ab170940)
  • Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody [EPR8982] (ab170940)
  • Anti-TPR antibody [EPR8982] (ab170940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8982] to TPR
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-TPR antibody [EPR8982]
    See all TPR primary antibodies

  • Description

    Rabbit monoclonal [EPR8982] to TPR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human TPR (Cysteine residue). The exact sequence is proprietary.
    Database link: P12270

  • Positive control

    • HeLa + Camptothecin, HeLa, HeLa + Staurosporine, HeLa, 293T and Jurkat whole cell lysate (ab7899), HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab170940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 267 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.

    • Tissue specificity

      Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.

    • Involvement in disease

      Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
      Note=Involved in tumorigenic rearrangements with the MET or RAF genes.

    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • Cellular localization

      Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
    • Information by UniProt

    • Database links

      • Alternative names

        • Nucleoprotein TPR antibody
        • tpr antibody
        • TPR_HUMAN antibody
        • translocated promoter region (to activated MET oncogene) antibody

      Images

      • Western blot - Anti-TPR antibody [EPR8982] (ab170940)
        Western blot - Anti-TPR antibody [EPR8982] (ab170940)
        All lanes : Anti-TPR antibody [EPR8982] (ab170940) at 1/1000 dilution

        Lane 1 : HeLa + Camptothecin cell lysates
        Lanes 2 & 4 : HeLa cell lysates
        Lane 3 : HeLa + Staurosporine cell lysates
        Lane 5 : 293T cell lysates
        Lane 6 : Jurkat cell lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 267 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody [EPR8982] (ab170940)
        Immunocytochemistry/ Immunofluorescence - Anti-TPR antibody [EPR8982] (ab170940)

        Immunofluorescence analysis of HeLa cells labeling TPR with ab170940 at 1/100 dilution.

      • Anti-TPR antibody [EPR8982] (ab170940)
        Anti-TPR antibody [EPR8982] (ab170940)

      References (1)

      Publishing research using ab170940? Please let us know so that we can cite the reference in this datasheet.

      ab170940 has been referenced in 1 publication.

      • Myers KN  et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . PubMed: 27739501

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