Overview

  • Product name
    Anti-TPR antibody [EPR8982]
    See all TPR primary antibodies
  • Description
    Rabbit monoclonal [EPR8982] to TPR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human TPR (Cysteine residue). The exact sequence is proprietary.
    Database link: P12270

  • Positive control
    • HeLa + Camptothecin, HeLa, HeLa + Staurosporine, HeLa, 293T and Jurkat cell lysates, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 267 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Component of the cytoplasmic fibrils of the nuclear pore complex implicated in nuclear protein import. Its N-terminus is involved in activation of oncogenic kinases. Plays a role in the mitotic spindle checkpoint.
    • Tissue specificity
      Highest in testis, lung, thymus, spleen and brain, lower levels in heart, liver and kidney.
    • Involvement in disease
      Defects in TPR are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=Chromosomal aberrations involving TPR are found in thyroid papillary carcinomas. Intrachromosomal rearrangement that links the 5'-end of the TPR gene to the protein kinase domain of NTRK1 forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the carboxy terminus of the NTRK1 protein.
      Note=Involved in tumorigenic rearrangements with the MET or RAF genes.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nuclear pore complex. Nucleus membrane. Chromosome > centromere > kinetochore. The assembly of the NPC is a stepwise process in which Trp-containing peripheral structures assemble after other components, including p62. Detected at kinetochores during prometaphase.
    • Information by UniProt
    • Database links
    • Alternative names
      • Nucleoprotein TPR antibody
      • tpr antibody
      • TPR_HUMAN antibody
      • translocated promoter region (to activated MET oncogene) antibody

    Images

    • All lanes : Anti-TPR antibody [EPR8982] (ab170940) at 1/1000 dilution

      Lane 1 : HeLa + Camptothecin cell lysates
      Lanes 2 & 4 : HeLa cell lysates
      Lane 3 : HeLa + Staurosporine cell lysates
      Lane 5 : 293T cell lysates
      Lane 6 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 267 kDa

    • Immunofluorescence analysis of HeLa cells labeling TPR with ab170940 at 1/100 dilution.

    References

    This product has been referenced in:
    • Myers KN  et al. The bornavirus-derived human protein EBLN1 promotes efficient cell cycle transit, microtubule organisation and genome stability. Sci Rep 6:35548 (2016). WB ; Human . Read more (PubMed: 27739501) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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