Recombinant Anti-TPST2 antibody [EPR10569] (ab157191)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10569] to TPST2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TPST2 antibody [EPR10569]
See all TPST2 primary antibodies -
Description
Rabbit monoclonal [EPR10569] to TPST2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human TPST2 aa 300 to the C-terminus.
Database link: O60704 -
Positive control
- Human fetal heart and liver lysates; Human uterus tissue and Human breast carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10569 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein sulfotransferase family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 8459 Human
- Entrez Gene: 22022 Mouse
- Entrez Gene: 288719 Rat
- Omim: 603126 Human
- SwissProt: O60704 Human
- SwissProt: O88856 Mouse
- SwissProt: Q5RJS8 Rat
- Unigene: 726122 Human
see all -
Alternative names
- EC 2.8.2.20 antibody
- Protein tyrosine sulfotransferase 2 antibody
- Protein-tyrosine sulfotransferase 2 antibody
see all
Images
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All lanes : Anti-TPST2 antibody [EPR10569] (ab157191) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TPST2 with ab157191 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TPST2 with ab157191 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab157191 has been referenced in 2 publications.
- Liu R et al. Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. Biotechnol J N/A:e2100142 (2021). PubMed: 34081410
- Scurci I et al. CCR5 tyrosine sulfation heterogeneity generates cell surface receptor subpopulations with different ligand binding properties. Biochim Biophys Acta Gen Subj 1865:129753 (2021). PubMed: 32991968