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    tpst2-antibody-epr10569-ab157191.pdf

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RecombinantRabMAb

Recombinant Anti-TPST2 antibody [EPR10569] (ab157191)

  • Datasheet
  • SDS
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Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
  • Anti-TPST2 antibody [EPR10569] (ab157191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10569] to TPST2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Protein
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Recombinant Human TPST2 protein (denatured) (ab156339)

View more associated products

Overview

  • Product name

    Anti-TPST2 antibody [EPR10569]
    See all TPST2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10569] to TPST2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human TPST2 aa 300 to the C-terminus.
    Database link: O60704

  • Positive control

    • Human fetal heart and liver lysates; Human uterus tissue and Human breast carcinoma tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10569
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Alternative Versions

    • Anti-TPST2 antibody [EPR10569] - BSA and Azide free (ab249299)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TPST2 protein (denatured) (ab156339)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab157191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

  • Function

    Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the protein sulfotransferase family.
  • Cellular localization

    Golgi apparatus membrane.
  • Target information above from: UniProt accession O60704 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8459 Human
    • Entrez Gene: 22022 Mouse
    • Entrez Gene: 288719 Rat
    • Omim: 603126 Human
    • SwissProt: O60704 Human
    • SwissProt: O88856 Mouse
    • SwissProt: Q5RJS8 Rat
    • Unigene: 726122 Human
    • Unigene: 250631 Mouse
    • Unigene: 3659 Rat
    see all
  • Alternative names

    • EC 2.8.2.20 antibody
    • Protein tyrosine sulfotransferase 2 antibody
    • Protein-tyrosine sulfotransferase 2 antibody
    • TANGO13B antibody
    • TPST-2 antibody
    • Tpst2 antibody
    • TPST2_HUMAN antibody
    • Transport and golgi organization 13 homolog B antibody
    • Tyrosylprotein phosphotransferase 2 antibody
    • Tyrosylprotein sulfotransferase 2 antibody
    see all

Images

  • Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)
    Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)
    All lanes : Anti-TPST2 antibody [EPR10569] (ab157191) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)

    Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TPST2 with ab157191 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TPST2 with ab157191 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-TPST2 antibody [EPR10569] (ab157191)
    Anti-TPST2 antibody [EPR10569] (ab157191)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab157191? Please let us know so that we can cite the reference in this datasheet.

ab157191 has been referenced in 2 publications.

  • Liu R  et al. Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. Biotechnol J N/A:e2100142 (2021). PubMed: 34081410
  • Scurci I  et al. CCR5 tyrosine sulfation heterogeneity generates cell surface receptor subpopulations with different ligand binding properties. Biochim Biophys Acta Gen Subj 1865:129753 (2021). PubMed: 32991968

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