Recombinant Anti-TPST2 antibody [EPR10569] (ab157191)
![Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)](https://www.abcam.com/ps/products/157/ab157191/Images/ab157191-164345-anti-tpst2-antibody-epr10569-western-blot.jpg "Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10569] to TPST2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TPST2 antibody [EPR10569]
See all TPST2 primary antibodies -
Description
Rabbit monoclonal [EPR10569] to TPST2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human TPST2 aa 300 to the C-terminus.
Database link: O60704 -
Positive control
- Human fetal heart and liver lysates; Human uterus tissue and Human breast carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10569 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 42 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein sulfotransferase family. -
Cellular localization
Golgi apparatus membrane. - Information by UniProt
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Database links
- Entrez Gene: 8459 Human
- Entrez Gene: 22022 Mouse
- Entrez Gene: 288719 Rat
- Omim: 603126 Human
- SwissProt: O60704 Human
- SwissProt: O88856 Mouse
- SwissProt: Q5RJS8 Rat
- Unigene: 726122 Human
see all -
Alternative names
- EC 2.8.2.20 antibody
- Protein tyrosine sulfotransferase 2 antibody
- Protein-tyrosine sulfotransferase 2 antibody
see all
Images
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Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)All lanes : Anti-TPST2 antibody [EPR10569] (ab157191) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)](https://www.abcam.com/ps/products/157/ab157191/Images/ab157191-164344-anti-tpst2-antibody-epr10569-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TPST2 with ab157191 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)](https://www.abcam.com/ps/products/157/ab157191/Images/ab157191-164343-anti-tpst2-antibody-epr10569-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TPST2 with ab157191 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-TPST2 antibody [EPR10569] (ab157191)](https://www.abcam.com/ps/products/157/ab157191/Images/ab157191-4-benefits-of-recombinant-antibodies.png)
Anti-TPST2 antibody [EPR10569] (ab157191)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab157191 has been referenced in 2 publications.
- Liu R et al. Modulating tyrosine sulfation of recombinant antibodies in CHO cell culture by host selection and sodium chlorate supplementation. Biotechnol J N/A:e2100142 (2021). PubMed: 34081410
- Scurci I et al. CCR5 tyrosine sulfation heterogeneity generates cell surface receptor subpopulations with different ligand binding properties. Biochim Biophys Acta Gen Subj 1865:129753 (2021). PubMed: 32991968