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RecombinantRabMAb

Recombinant Anti-TPST2 antibody [EPR10569] (ab157191)

  • Datasheet
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Abpromise

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Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
  • Anti-TPST2 antibody [EPR10569] (ab157191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10569] to TPST2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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Protein
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Recombinant Human TPST2 protein (denatured) (ab156339)

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Overview

  • Product name

    Anti-TPST2 antibody [EPR10569]
    See all TPST2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10569] to TPST2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human TPST2 aa 300 to the C-terminus.
    Database link: O60704

  • Positive control

    • Human fetal heart and liver lysates; Human uterus tissue and Human breast carcinoma tissues.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10569
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Other

Associated products

  • Alternative Versions

    • Anti-TPST2 antibody [EPR10569] - BSA and Azide free (ab249299)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human TPST2 protein (denatured) (ab156339)

Applications

Our Abpromise guarantee covers the use of ab157191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the protein sulfotransferase family.
    • Cellular localization

      Golgi apparatus membrane.
    • Target information above from: UniProt accession O60704 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8459 Human
      • Entrez Gene: 22022 Mouse
      • Entrez Gene: 288719 Rat
      • Omim: 603126 Human
      • SwissProt: O60704 Human
      • SwissProt: O88856 Mouse
      • SwissProt: Q5RJS8 Rat
      • Unigene: 726122 Human
      • Unigene: 731748 Human
      • Unigene: 250631 Mouse
      • Unigene: 3659 Rat
      see all
    • Alternative names

      • EC 2.8.2.20 antibody
      • Protein tyrosine sulfotransferase 2 antibody
      • Protein-tyrosine sulfotransferase 2 antibody
      • TANGO13B antibody
      • TPST-2 antibody
      • Tpst2 antibody
      • TPST2_HUMAN antibody
      • Transport and golgi organization 13 homolog B antibody
      • Tyrosylprotein phosphotransferase 2 antibody
      • Tyrosylprotein sulfotransferase 2 antibody
      see all

    Images

    • Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)
      Western blot - Anti-TPST2 antibody [EPR10569] (ab157191)
      All lanes : Anti-TPST2 antibody [EPR10569] (ab157191) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling TPST2 with ab157191 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPST2 antibody [EPR10569] (ab157191)

      Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling TPST2 with ab157191 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-TPST2 antibody [EPR10569] (ab157191)
      Anti-TPST2 antibody [EPR10569] (ab157191)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab157191? Please let us know so that we can cite the reference in this datasheet.

    ab157191 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

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