Key features and details
- Rabbit polyclonal to TPX2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TPX2 antibody
See all TPX2 primary antibodies
DescriptionRabbit polyclonal to TPX2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residue 700 and the C-terminus (residue 749) of Human TPX2 (Swiss-Prot Q9ULW0).
- HeLa whole cell lysate (ab150035)
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/15000. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).|
|IP||Use a concentration of 1 - 4 µg/ml.|
FunctionSpindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Tissue specificityExpressed in lung carcinoma cell lines but not in normal lung tissues.
Sequence similaritiesBelongs to the TPX2 family.
Developmental stageExclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.
- Information by UniProt
- C20ORF1 antibody
- C20orf2 antibody
- Chromosome 20 Open Reading Frame 1 antibody
All lanes : Anti-TPX2 antibody (ab70237) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 5 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 86 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab70237 has been referenced in 2 publications.
- Jiang T et al. MiR-29a-5p inhibits proliferation and invasion and induces apoptosis in endometrial carcinoma via targeting TPX2. Cell Cycle 17:1268-1278 (2018). PubMed: 29888640
- Hebras C & McDougall A Urochordate Ascidians Possess a Single Isoform of Aurora Kinase That Localizes to the Midbody via TPX2 in Eggs and Cleavage Stage Embryos. PLoS One 7:e45431 (2012). ICC/IF . PubMed: 23029005