Key features and details
- Rabbit polyclonal to TR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TR1 antibody
See all TR1 primary antibodies
DescriptionRabbit polyclonal to TR1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human TR1 aa 129-223 (N terminal).
Database link: Q7RTX1
- A549, HeLa, Jurkat and NT2D1 cell lysates; HeLa cells; Human CL1-0 xenograft tissue.
This product was previously labelled as GPCR TAS1R1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab155143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 93 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionPutative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.
Sequence similaritiesBelongs to the G-protein coupled receptor 3 family. TAS1R subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- G protein coupled receptor 70 antibody
- G protein-coupled receptor 70 antibody
- G-protein coupled receptor 70 antibody
Anti-TR1 antibody (ab155143) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin embedded Human CL1-0 xenograft tissue, labeling TR1 using ab155143 at a 1/100 dilution.
Immunofluorescence analysis of methanol fixed HeLa cells, labeling TR1 using ab155143 at a 1/200 dilution. Lower image costained with Hoerchst 33342.
ab155143 has been referenced in 1 publication.
- Zhou Y et al. Methionine Regulates mTORC1 via the T1R1/T1R3-PLCß-Ca(2+)-ERK1/2 Signal Transduction Process in C2C12 Cells. Int J Mol Sci 17:N/A (2016). WB ; Mouse . PubMed: 27727170