Overview

  • Product name

    Anti-TR2-11 antibody
  • Description

    Rabbit polyclonal to TR2-11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ATIEEIAHQI IEQQMGEIVT EQQTGQKIQI VTALDHNTQG KQFILTNHDG) of Human TR2-11 (NP_001027458).

  • Positive control

    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function

    Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression.
    Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB.
  • Cellular localization

    Nucleus. Nucleus > PML body. Recruited by HDAC3, after all-trans retinoic acid stimulated MAPK1-mediated Thr-223 phosphorylation, to PML bodies for subsequent sumoylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • NR2C 1 antibody
    • NR2C1 antibody
    • NR2C1_HUMAN antibody
    • NUCLEAR HORMONE RECEPTOR TR2 antibody
    • Nuclear receptor subfamily 2 Group C isoform antibody
    • Nuclear receptor subfamily 2 group C member 1 antibody
    • Orphan nuclear receptor TR2 antibody
    • Testicular receptor 2 antibody
    • TR 2 antibody
    • TR2 antibody
    see all

Images

  • Anti-TR2-11 antibody (ab83278) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 51 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labelling TR2-11 with ab83278 at 5.0µg/ml.

References

ab83278 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Schwann cells)
Loading amount
50 µg
Specification
Primary Schwann cells
Gel Running Conditions
Non-reduced Denaturing (10% Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Laura Rosenberg

Verified customer

Submitted Feb 15 2012

Question
Answer

Thank you for contacting us. As discussed over the phone we do not do sample sizes of our antibodies due to the size of our catalogue unfortunately it just isn't logistically feasible. We do however do a testing discount. As you mentioned, ab83278 has not been used with rat samples but as the immunogen shares 92% homology with the Rat TR2-11 we would predict that it would react. I have therefore generated the testing discount below: which is valid until 6th of March, 2012 In order to redeem this offer, please purchase ab83278 as normal, test it with your rat samples with Western blotting or IP, then please submit an Abreview and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews As discussed, we publish both positive and negative Abreviews on our datasheets and you would be eligable for your free primary antibody either way so please submit the results of your tests whatever they are. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount  

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