Overview

  • Product name

  • Description

    Rabbit polyclonal to TR4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human TR4 aa 62-356.
    Database link: P49116

  • Positive control

    • HeLa, H1299 and Molt-4 cells. NCIN87 Xenograft.
  • General notes

    Previously labelled as NR2C2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab137582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 65 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stage

    Transiently repressed during the meiotic phase of spermatogenesis.
  • Post-translational
    modifications

    Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • hTAK1 antibody
    • Nr2c2 antibody
    • NR2C2_HUMAN antibody
    • Nuclear hormone receptor TR4 antibody
    • Nuclear receptor subfamily 2 group C member 2 antibody
    • Orphan nuclear receptor TAK1 antibody
    • Orphan nuclear receptor TR4 antibody
    • TAK1 antibody
    • Testicular nuclear receptor 4 antibody
    • Testicular receptor 4 antibody
    • TR2R1 antibody
    • TR4 antibody
    • TR4 nuclear hormone receptor antibody
    see all

Images

  • All lanes : Anti-TR4 antibody (ab137582) at 1/500 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : Molt-4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 65 kDa



    7.5% SDS-PAGE
  • Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft labelling TR4 with ab137582 at 1/100 dilution.

  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labelling TR4 with ab137582 at 1/500 dilution.

References

This product has been referenced in:

  • Zhang Y  et al. Role and mechanism of miR-4778-3p and its targets NR2C2 and Med19 in cervical cancer radioresistance. Biochem Biophys Res Commun 508:210-216 (2019). Read more (PubMed: 30473219) »
See 1 Publication for this product

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