Product nameAnti-TR4 antibody [EPR1772(2)]
See all TR4 primary antibodies
DescriptionRabbit monoclonal [EPR1772(2)] to TR4
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human TR4 aa 1-100. The exact sequence is proprietary.
- 293T, PC-3 and U937 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as NR2C2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).|
|IP||1/10 - 1/100.|
FunctionOrphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Developmental stageTransiently repressed during the meiotic phase of spermatogenesis.
modificationsPhosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- hTAK1 antibody
- Nr2c2 antibody
- NR2C2_HUMAN antibody
All lanes : Anti-TR4 antibody [EPR1772(2)] (ab109513) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : U937 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
ab109513 has not yet been referenced specifically in any publications.