Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1772(2)] to TR4 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
Product nameAnti-TR4 antibody [EPR1772(2)] - BSA and Azide free
See all TR4 primary antibodies
DescriptionRabbit monoclonal [EPR1772(2)] to TR4 - BSA and Azide free
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human TR4 aa 1-100. The exact sequence is proprietary.
ab247887 is the carrier-free version of ab109513 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247887 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab247887 in the following tested applications.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).|
FunctionOrphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Developmental stageTransiently repressed during the meiotic phase of spermatogenesis.
modificationsPhosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- hTAK1 antibody
- Nr2c2 antibody
- NR2C2_HUMAN antibody
All lanes : Anti-TR4 antibody [EPR1772(2)] (ab109513) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : U937 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 65 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
This data was developed using ab109513, the same antibody clone in a different buffer formulation.
ab247887 has not yet been referenced specifically in any publications.