RecombinantRabMAb

Recombinant Anti-TR4 antibody [EPR1773(2)] (ab109301)

Submit a review Submit a question References (6)
Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
  • Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)
  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
  • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
  • Anti-TR4 antibody [EPR1773(2)] (ab109301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1773(2)] to TR4
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TR4 antibody [EPR1773(2)]
    See all TR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR1773(2)] to TR4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human TR4 aa 500 to the C-terminus.

  • Positive control

    • PC3 cell lysate, 293T cell lysate; Human testis tissue

  • General notes

     

     

    Previously labelled as NR2C2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1773(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Developmental stage

      Transiently repressed during the meiotic phase of spermatogenesis.

    • Post-translational
      modifications

      Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Form

      There are 2 isoforms produced by alternative splicing.

    • Alternative names

      • hTAK1 antibody
      • Nr2c2 antibody
      • NR2C2_HUMAN antibody
      • Nuclear hormone receptor TR4 antibody
      • Nuclear receptor subfamily 2 group C member 2 antibody
      • Orphan nuclear receptor TAK1 antibody
      • Orphan nuclear receptor TR4 antibody
      • TAK1 antibody
      • Testicular nuclear receptor 4 antibody
      • Testicular receptor 4 antibody
      • TR2R1 antibody
      • TR4 antibody
      • TR4 nuclear hormone receptor antibody
      see all

    Images

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution

      Lane 1 : Wild-type HEK293T cell lysate
      Lane 2 : NR2C2 knockout HEK293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa
      why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab109301 observed at 67 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab109301 Anti-TR4 antibody [EPR1773(2)] was shown to specifically react with TR4 in wild-type HEK293T cells. The band observed in knockout cell line ab266228 (knockout cell lysate ab257563) lane below 67 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and TR4 knockout samples were subjected to SDS-PAGE. ab109301 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)

      ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)

      Lane 1 : PC-12 whole cell lysate
      Lane 2 : 3T3-L1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK293 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution (Unpurified)

      Lane 1 : PC3 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    • Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Anti-TR4 antibody [EPR1773(2)] (ab109301)

    References (6)

    Publishing research using ab109301? Please let us know so that we can cite the reference in this datasheet.

    ab109301 has been referenced in 6 publications.

    • Shi H  et al. Targeting the TR4 nuclear receptor-mediated lncTASR/AXL signaling with tretinoin increases the sunitinib sensitivity to better suppress the RCC progression. Oncogene 39:530-545 (2020). PubMed: 31501521
    • Hu L  et al. Targeting TR4 nuclear receptor with antagonist bexarotene increases docetaxel sensitivity to better suppress the metastatic castration-resistant prostate cancer progression. Oncogene 39:1891-1903 (2020). PubMed: 31748715
    • Chen Y  et al. Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma. Cancer Sci 109:384-394 (2018). PubMed: 29197138
    • Wang M  et al. Preclinical studies using miR-32-5p to suppress clear cell renal cell carcinoma metastasis via altering the miR-32-5p/TR4/HGF/Met signaling. Int J Cancer 143:100-112 (2018). PubMed: 29396852
    • Al-Qahtani SM  et al. 17ß-Estradiol suppresses visceral adipogenesis and activates brown adipose tissue-specific gene expression. Horm Mol Biol Clin Investig 29:13-26 (2017). PubMed: 27831918
    • Zhang D  et al. Targeting the ERK pathway for the treatment of Cushing's disease. Oncotarget 7:69149-69158 (2016). PubMed: 27708250

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