Recombinant
RabMAb

Recombinant Anti-TR4 antibody [EPR1773(2)] (ab109301)

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Overview

  • Product name

    Anti-TR4 antibody [EPR1773(2)]
    See all TR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR1773(2)] to TR4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    corresponding to Human TR4 aa 500 to the C-terminus.

  • Positive control

    • PC3 cell lysate, 293T cell lysate; Human testis tissue

  • General notes

     

     

    Previously labelled as NR2C2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1773(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.

    • Sequence similarities

      Belongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • Developmental stage

      Transiently repressed during the meiotic phase of spermatogenesis.

    • Post-translational
      modifications

      Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Form

      There are 2 isoforms produced by alternative splicing.

    • Alternative names

      • hTAK1 antibody
      • Nr2c2 antibody
      • NR2C2_HUMAN antibody
      • Nuclear hormone receptor TR4 antibody
      • Nuclear receptor subfamily 2 group C member 2 antibody
      • Orphan nuclear receptor TAK1 antibody
      • Orphan nuclear receptor TR4 antibody
      • TAK1 antibody
      • Testicular nuclear receptor 4 antibody
      • Testicular receptor 4 antibody
      • TR2R1 antibody
      • TR4 antibody
      • TR4 nuclear hormone receptor antibody
      see all

    Images

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified)

      Lane 1 : PC-12 whole cell lysate
      Lane 2 : 3T3-L1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/5000 dilution (purified) + HEK293 whole cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/2000 dilution (purified) + PC-3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Immunoprecipitation - Anti-TR4 antibody [EPR1773(2)] (ab109301)

      ab109301 (purified) at 1/30 immunoprecipitating TR4 in 10 μg HEK293 cell lysate (Lanes 1 and 2, observed at 67 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      Western blot - Anti-TR4 antibody [EPR1773(2)] (ab109301)
      All lanes : Anti-TR4 antibody [EPR1773(2)] (ab109301) at 1/1000 dilution (Unpurified)

      Lane 1 : PC3 cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 65 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Chen Y  et al. Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma. Cancer Sci 109:384-394 (2018). Read more (PubMed: 29197138) »
    • Wang M  et al. Preclinical studies using miR-32-5p to suppress clear cell renal cell carcinoma metastasis via altering the miR-32-5p/TR4/HGF/Met signaling. Int J Cancer 143:100-112 (2018). Read more (PubMed: 29396852) »
    See all 3 Publications for this product

    Publishing research using ab109301? Please let us know so that we can cite the reference in this datasheet.

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