Product nameAnti-TRA-1-60 (R) antibody [TRA-1-60]
DescriptionMouse monoclonal [TRA-1-60] to TRA-1-60 (R)
The TRA-1-60 monoclonal binds to a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010). Two subclones of the TRA-1-60 hybridoma were found to be either sensitive (S) or resistant (R) to neuraminidase treatment but both recognize the target similarly (PubMed ID: 21651578).
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IP, ICC, RIA, IHC-Frmore details
Species reactivityReacts with: Rabbit, Human
Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
PurityTissue culture supernatant
Purification notesTissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
Our Abpromise guarantee covers the use of ab16288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20674000|
|Flow Cyt||Use a concentration of 10 - 20 µg/ml.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).|
|IP||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceTRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010).
Cellular localizationCell Membrane
- TRA 1 60 antibody
ab16288 staining TRA-160 (R) in HUES7 cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1/500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
ab16288 staining TRA-1-60 (R) in Human H9 cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100. Cells were incubated with the primary antibody (1/1000) for 1 hour at 4°C. Gating Strategy: Unstained H9 cells.
Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1/500 dilution.
This product has been referenced in:
- Grigor'eva EV et al. Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease. Stem Cell Res 34:101382 (2019). Read more (PubMed: 30658253) »
- Vilà-González M et al. Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling. Stem Cells 37:226-239 (2019). Read more (PubMed: 30372556) »