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Stem Cells Embryonic Stem Cells Surface Molecules
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Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

  • Datasheet
  • SDS
Reviews (8)Q&A (1)References (74)

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Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

    Key features and details

    • Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgM

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    Overview

    • Product name

      Anti-TRA-1-60 (R) antibody [TRA-1-60]
    • Description

      Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Rabbit
    • Immunogen

      Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.

    • General notes

      This antibody clone is manufactured by Abcam.

      If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 6.97% L-Arginine
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Purification notes

      Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
    • Clonality

      Monoclonal
    • Clone number

      TRA-1-60
    • Myeloma

      P3x66Ag.8-Sp2/0
    • Isotype

      IgM
    • Research areas

      • Stem Cells
      • Embryonic Stem Cells
      • Surface Molecules
      • Developmental Biology
      • Embryogenesis
      • Embryonic stem cells
      • Intracellular

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Isotype control

      • Mouse IgM [B11/7] - Isotype control (ab91545)
    • Related Products

      • Anti-SSEA4 antibody [MC813-70] (ab16287)
      • Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab16288 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (1)
    Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
    Notes
    WB
    Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).

    Target

    • Relevance

      TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010).
    • Cellular localization

      Cell Membrane
    • Alternative names

      • TRA 1 60 antibody

    Images

    • Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
      Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
      All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml

      Lane 1 : Human Embryonic Stem Cell Lysate
      Lane 2 : Mouse Embryonic Stem Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution

      Predicted band size: 235 kDa
      Observed band size: 250 kDa why is the actual band size different from the predicted?



      As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.

    Protocols

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (74)

    Publishing research using ab16288? Please let us know so that we can cite the reference in this datasheet.

    ab16288 has been referenced in 74 publications.

    • Liu M  et al. Small extracellular vesicles derived from embryonic stem cells restore ovarian function of premature ovarian failure through PI3K/AKT signaling pathway. Stem Cell Res Ther 11:3 (2020). PubMed: 31900201
    • Schwarz N  et al. Establishment of a human induced pluripotent stem cell (iPSC) line (HIHDNEi002-A) from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Arg297Gln) mutation. Stem Cell Res 37:101445 (2019). PubMed: 31075689
    • Guo Y  et al. Modeling Retinitis Pigmentosa: Retinal Organoids Generated From the iPSCs of a Patient With the USH2A Mutation Show Early Developmental Abnormalities. Front Cell Neurosci 13:361 (2019). PubMed: 31481876
    • DiSalvo M  et al. Automated sensing and splitting of stem cell colonies on microraft arrays. APL Bioeng 3:036106 (2019). PubMed: 31489396
    • Li G  et al. Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations. Front Mol Neurosci 12:212 (2019). PubMed: 31572124
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human iPSC cells)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    human iPSC cells
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Herr Dr. Vladimir Milenkovic

    Verified customer

    Submitted Nov 23 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (embryonic stem cell)
    Permeabilization
    No
    Specification
    embryonic stem cell
    Blocking step
    BSA as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kate Hawkins

    Verified customer

    Submitted May 17 2016

    Flow Cytometry abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (H9 hESCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Fibroblasts (white)
    Specification
    H9 hESCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

    Flow Cytometry abreview for Anti-TRA-1-60 (R) [TRA-1-60] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Sample
    Human Cell (H9 human)
    Specification
    H9 human
    Gating Strategy
    Unstained H9 cells
    Permeabilization
    Yes - 0.2% triton x-100
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 12 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Human Cell (H9 human)
    Specification
    H9 human
    Permeabilization
    Yes - 0.03% TritonX-100
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 11 2013

    Western blot abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Cell lysate - whole cell (H9 embryonic stem cells)
    Specification
    H9 embryonic stem cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 10 2013

    Question

    I was looking for the Tra-1-60 antibody of yours and its specificity. The western-blot data shows discrepancy with figure and its legend. The legends states that the antibody needs to react with Human embryonic stem cells, whereas the data shows the reactivity with mouse embryonic stem cells. Please make the necessary changes.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 10 2009

    Answer

    Thank you for contacting us. I have checked the WB image information and indeed the figure and the legend information are contradicting. Based on the information that we have in the system, it seems that the WB Lane information was mixed-up and I sincerely apologize for the error. Please rest assured that I have now rectified the mistake and have updated the datasheet. It should now state: Lane 1 : Human Embryonic Stem Cell Lysate Lane 2 : Mouse Embryonic Stem Cell Lysate Thank you again for pointing this out to us. We always strive to provide accurate and honest information on all of our products but sometimes do make human error. It is good to know that we have loyal customers like you to point this out if it occurs. Should you have any questions related to our products, please always feel free to contact us. Have a nice day!

    Read More

    Abcam Scientific Support

    Answered on Sep 10 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Embryonic Stem Cell, HUES7)
    Specification
    Embryonic Stem Cell, HUES7
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Ms. Fiona Lewis

    Verified customer

    Submitted Aug 05 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (embryonic stem cells)
    Specification
    embryonic stem cells
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5%
    Read More

    Dr. Maria Keramari

    Verified customer

    Submitted Dec 06 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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