Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
- Datasheet
- References (67)
- Protocols
Overview
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Product name
Anti-TRA-1-60 (R) antibody [TRA-1-60] -
Description
Mouse monoclonal [TRA-1-60] to TRA-1-60 (R) -
Host species
Mouse -
Specificity
The TRA-1-60 monoclonal binds to a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010). Two subclones of the TRA-1-60 hybridoma were found to be either sensitive (S) or resistant (R) to neuraminidase treatment but both recognize the target similarly (PubMed ID: 21651578).
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Tested applications
Suitable for: ICC/IF, Flow Cyt, WB, IP, ICC, RIA, IHC-Frmore details -
Species reactivity
Reacts with: Rabbit, Human -
Immunogen
Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
Clonality
Monoclonal -
Clone number
TRA-1-60 -
Myeloma
P3x66Ag.8-Sp2/0 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab16288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 20674000 | |
| Flow Cyt | Use a concentration of 10 - 20 µg/ml. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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| WB | Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa). | |
| IP | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| RIA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
Target
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Relevance
TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010). -
Cellular localization
Cell Membrane -
Alternative names
- TRA 1 60 antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-24937-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an Abreview submitted by Ms Fiona Lewisab16288 staining TRA-160 (R) in HUES7 cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1/500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
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![Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-24939-anti-tra-1-60-r-antibody-tra-1-60-western-blot.jpg)
Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody. -
![Flow Cytometry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-203464-anti-tra-1-60-r-antibody-tra-1-60-flow-cytometry.jpg)
Flow Cytometry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an anonymous Abreviewab16288 staining TRA-1-60 (R) in Human H9 cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100. Cells were incubated with the primary antibody (1/1000) for 1 hour at 4°C. Gating Strategy: Unstained H9 cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-272266-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, GermanyImmunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1/500 dilution.
Protocols
References
This product has been referenced in:
- Grigor'eva EV et al. Generation of induced pluripotent stem cell line, ICGi007-A, by reprogramming peripheral blood mononuclear cells from a patient with Huntington's disease. Stem Cell Res 34:101382 (2019). Read more (PubMed: 30658253) »
- Vilà-González M et al. Enhanced Function of Induced Pluripotent Stem Cell-Derived Endothelial Cells Through ESM1 Signaling. Stem Cells 37:226-239 (2019). Read more (PubMed: 30372556) »
