Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
![Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-24939-anti-tra-1-60-r-antibody-tra-1-60-western-blot.jpg "Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)")
Key features and details
- Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
- Suitable for: IP, ICC, RIA, IHC-Fr, ICC/IF, Flow Cyt, WB
- Reacts with: Rabbit, Human
- Isotype: IgM
Overview
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Product name
Anti-TRA-1-60 (R) antibody [TRA-1-60] -
Description
Mouse monoclonal [TRA-1-60] to TRA-1-60 (R) -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC, RIA, IHC-Fr, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Rabbit, Human -
Immunogen
Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.
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Positive control
- WB: Human embryonic stem cell lysate. ICC: Human embryonic stem cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
Clonality
Monoclonal -
Clone number
TRA-1-60 -
Myeloma
P3x66Ag.8-Sp2/0 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| ICC |
1/500.
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| RIA |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| ICC/IF | (5) |
Use at an assay dependent concentration. PubMed: 20674000
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| Flow Cyt | (2) |
Use at an assay dependent concentration.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
| WB | (1) |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
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| Notes |
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IP
Use at an assay dependent concentration. |
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ICC
1/500. |
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RIA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 20674000 |
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Flow Cyt
Use at an assay dependent concentration. ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
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WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa). |
Target
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Relevance
TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010). -
Cellular localization
Cell Membrane -
Alternative names
- TRA 1 60 antibody
Images
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Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody. -
![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-24937-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an Abreview submitted by Ms Fiona LewisImmunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C. Samples were incubated with primary antibody, dilution 1:500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgG, dilution 1:100, was used as secondary antibody.
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![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-470577-anti-tra-1-60-r-antibody-tra-1-60-immunocytochemistry.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an anonymous collaborator.This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.
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![Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-272266-anti-tra-1-60-r-antibody-tra-1-60-immunofluorescence.jpg)
Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, GermanyImmunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1:500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1:500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (104)
ab16288 has been referenced in 104 publications.
- Arioka Y et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons. EBioMedicine 63:103138 (2021). PubMed: 33341442
- Sarvestani SK et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun 12:262 (2021). PubMed: 33431859
- Liang F et al. SORBS2 is a genetic factor contributing to cardiac malformation of 4q deletion syndrome patients. Elife 10:N/A (2021). PubMed: 34099102
- Martens YA et al. Generation and validation of APOE knockout human iPSC-derived cerebral organoids. STAR Protoc 2:100571 (2021). PubMed: 34151296
- Woods S et al. Generation of Human-Induced Pluripotent Stem Cells From Anterior Cruciate Ligament. J Orthop Res 38:92-104 (2020). PubMed: 31613026