Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

Abreviews (8)Q&A (1)Specific References (44)

Overview

  • Product name
    Anti-TRA-1-60 (R) antibody [TRA-1-60]
  • Description
    Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IP, ICC, RIA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Purity
    Tissue culture supernatant
  • Purification notes
    Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • Clonality
    Monoclonal
  • Clone number
    TRA-1-60
  • Myeloma
    P3x66Ag.8-Sp2/0
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20674000
Flow Cyt Use a concentration of 10 - 20 µg/ml.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    The monoclonal antibody TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The core protein recognised by TRA-1-60 has not been identified but the antibody is thought to recognise carbohydrate epitopes associated with a high molecular weight proteoglycan.
  • Cellular localization
    Cell Membrane
  • Alternative names
    • TRA 1 60 antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an Abreview submitted by Ms Fiona Lewis

    ab16288 staining TRA-160 (R) in HUES7 cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C.  Samples were incubated with primary antibody, dilution 1/500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.

    See Abreview

  • Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution

    Predicted band size: 235 kDa
    Observed band size: 250 kDa (why is the actual band size different from the predicted?)



    As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
  • Flow Cytometry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Flow Cytometry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an anonymous Abreview

    ab16288 staining TRA-1-60 (R) in Human H9 cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100. Cells were incubated with the primary antibody (1/1000) for 1 hour at 4°C. Gating Strategy: Unstained H9 cells.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Immunocytochemistry/ Immunofluorescence - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany
    Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1/500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1/500 dilution. 

    See Abreview

References

This product has been referenced in:
  • Gowran A  et al. Generation of induced pluripotent stem cells from a Becker muscular dystrophy patient carrying a deletion of exons 45-55 of the dystrophin gene (CCMi002BMD-A-9 ?45-55). Stem Cell Res 28:21-24 (2018). Flow Cyt . Read more (PubMed: 29414413) »
  • Wang Y  et al. Establishment of TUSMi003-A, an induced pluripotent stem cell (iPSC) line from a 62-year old Chinese Han patient with Alzheimer's disease with ApoE3/4 genetic background. Stem Cell Res 27:57-60 (2018). Read more (PubMed: 29328972) »

See all 44 Publications for this product

Publishing research using ab16288? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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9 reviews or Q&A

10
Votes: Highest First

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60]

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human iPSC cells)
Permeabilization
Yes - 0.5% TX100
Specification
human iPSC cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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Username

Herr Dr. Vladimir Milenkovic

Verified customer

Submitted Nov 23 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60]

 Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cell)
Permeabilization
No
Specification
embryonic stem cell
Blocking step
BSA as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde
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Username

Dr. Kate Hawkins

Verified customer

Submitted May 17 2016

Flow Cytometry abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60]

 Excellent
Abreviews
Application
Flow Cytometry
Sample
Human Cell (H9 hESCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Fibroblasts (white)
Specification
H9 hESCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
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Username

Abcam user community

Verified customer

Submitted Sep 09 2015

Flow Cytometry abreview for Anti-TRA-1-60 (R) [TRA-1-60] antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (H9 human)
Specification
H9 human
Gating Strategy
Unstained H9 cells
Permeabilization
Yes - 0.2% triton x-100
Read More
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Username

Abcam user community

Verified customer

Submitted Aug 12 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (H9 human)
Specification
H9 human
Permeabilization
Yes - 0.03% TritonX-100
Fixative
Paraformaldehyde
Read More
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Username

Abcam user community

Verified customer

Submitted Jul 11 2013

Western blot abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (H9 embryonic stem cells)
Specification
H9 embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Username

Abcam user community

Verified customer

Submitted Jul 10 2013

I was looking for the Tra-1-60 antibody of yours and its specificity. The western-blot data shows discrepancy with figure and its legend. The legends states that the antibody needs to react with Human embryonic stem cells, whereas the data shows the re...

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Thank you for contacting us. I have checked the WB image information and indeed the figure and the legend information are contradicting. Based on the information that we have in the system, it seems that the WB Lane information was mixed-up and I s...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Embryonic Stem Cell, HUES7)
Specification
Embryonic Stem Cell, HUES7
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Read More
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Username

Ms. Fiona Lewis

Verified customer

Submitted Aug 05 2009

Immunocytochemistry/ Immunofluorescence abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cells)
Specification
embryonic stem cells
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Read More
abreview image
Username

Dr. Maria Keramari

Verified customer

Submitted Dec 06 2006

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