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Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

Reviews (8)Q&A (1)References (105)
Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)

Key features and details

  • Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
  • Suitable for: ICC, WB
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-TRA-1-60 (R) antibody [TRA-1-60]

  • Description

    Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit

  • Immunogen

    Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.

  • Positive control

    • WB: Human embryonic stem cell lysate. ICC: Human embryonic stem cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS

  • Clonality

    Monoclonal

  • Clone number

    TRA-1-60

  • Myeloma

    P3x66Ag.8-Sp2/0

  • Isotype

    IgM

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/500.
WB (1)
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
Notes
ICC
1/500.
WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).

Target

  • Relevance

    TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010).

  • Cellular localization

    Cell Membrane

  • Alternative names

    • TRA 1 60 antibody

Images

  • Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate
    Lane 2 : Mouse Embryonic Stem Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution

    Predicted band size: 235 kDa
    Observed band size: 250 kDa why is the actual band size different from the predicted?



    As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an Abreview submitted by Ms Fiona Lewis

    Immunocytochemical analysis of ab16288 staining TRA-160 (R) in HUES7 cells. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked in 10% serum for 1 hour at 24°C.  Samples were incubated with primary antibody, dilution 1:500 (1% serum, 0.1% Triton in PBS), for 1 hour at 37°C. An Alexa Fluor® 647-conjugated goat polyclonal to mouse IgG, dilution 1:100, was used as secondary antibody.

    See Abreview

  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an anonymous collaborator.

    This image shows a colony of Human Embryonic Stem Cells stained with dapi (blue), anti-Oct4 antibody ab19857 (red) and Tra-1-60 antibody ab16288 (green). The nuclei of Oct4-positive undifferentiated hESCs stained bright red, whereas Tra-1-60 staining was seen at the cell membrane. These antibodies can be used as markers of undifferentiated Human Embryonic Stem Cells.

  • Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
    Immunocytochemistry - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany
    Immunocytochemistry analysis of human iPSC cells labeling TRA-1-60 (R) with ab16288 at 1:500 dilution. Cells were fixed with paraformaldehyde and permeabilized with 0.5% TX100. 5% serum was used to block cells for 20 minutes at 25°C. A polyclonal goat anti-mouse Cy3 secondary antibody was used at 1:500 dilution.

    See Abreview

References (105)

Publishing research using ab16288? Please let us know so that we can cite the reference in this datasheet.

ab16288 has been referenced in 105 publications.

  • Arioka Y  et al. Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons. EBioMedicine 63:103138 (2021). PubMed: 33341442
  • Sarvestani SK  et al. Induced organoids derived from patients with ulcerative colitis recapitulate colitic reactivity. Nat Commun 12:262 (2021). PubMed: 33431859
  • Liang F  et al. SORBS2 is a genetic factor contributing to cardiac malformation of 4q deletion syndrome patients. Elife 10:N/A (2021). PubMed: 34099102
  • Martens YA  et al. Generation and validation of APOE knockout human iPSC-derived cerebral organoids. STAR Protoc 2:100571 (2021). PubMed: 34151296
  • Woods S  et al. Generation of Human-Induced Pluripotent Stem Cells From Anterior Cruciate Ligament. J Orthop Res 38:92-104 (2020). PubMed: 31613026
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-TRA-1-60 (R) antibody [TRA-1-60] - Embryonic Stem Cell Marker

 Average
Abreviews
abreview image
Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (H9 embryonic stem cells)
Specification
H9 embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Jul 10 2013

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