Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)
![Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)](https://www.abcam.com/ps/products/16/ab16288/Images/ab16288-24939-anti-tra-1-60-r-antibody-tra-1-60-western-blot.jpg "Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)")
Key features and details
- Mouse monoclonal [TRA-1-60] to TRA-1-60 (R)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-TRA-1-60 (R) antibody [TRA-1-60] -
Description
Mouse monoclonal [TRA-1-60] to TRA-1-60 (R) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit
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Immunogen
Tissue, cells or virus corresponding to Human TRA-1-60 (R). Human embryonal carcinoma cell line 2102Ep cl.2A6.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
Clonality
Monoclonal -
Clone number
TRA-1-60 -
Myeloma
P3x66Ag.8-Sp2/0 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 235 kDa). |
Target
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Relevance
TRA-1-60 is a marker of Human Embryonic Stem, Germ and Carcinoma Cells but Not Mouse Embryonic Stem, Germ or Carcinoma Cells. The TRA-1-60 target has been reported to be a carbohydrate epitope associated with podocalyxin (PubMed IDs: 10493530, 17124010). -
Cellular localization
Cell Membrane -
Alternative names
- TRA 1 60 antibody
Images
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Western blot - Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288)All lanes : Anti-TRA-1-60 (R) antibody [TRA-1-60] (ab16288) at 10 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Predicted band size: 235 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
As expected, ab16288 recognizes a band in Human Embryonic Stem Cell Lysate but not Mouse Embryonic Stem Cell Lysate. The smear above the main band is due to protein glycosylation. The Western Blot profile is as expected for the TRA-1-60 monoclonal antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (74)
ab16288 has been referenced in 74 publications.
- Liu M et al. Small extracellular vesicles derived from embryonic stem cells restore ovarian function of premature ovarian failure through PI3K/AKT signaling pathway. Stem Cell Res Ther 11:3 (2020). PubMed: 31900201
- Schwarz N et al. Establishment of a human induced pluripotent stem cell (iPSC) line (HIHDNEi002-A) from a patient with developmental and epileptic encephalopathy carrying a KCNA2 (p.Arg297Gln) mutation. Stem Cell Res 37:101445 (2019). PubMed: 31075689
- Guo Y et al. Modeling Retinitis Pigmentosa: Retinal Organoids Generated From the iPSCs of a Patient With the USH2A Mutation Show Early Developmental Abnormalities. Front Cell Neurosci 13:361 (2019). PubMed: 31481876
- DiSalvo M et al. Automated sensing and splitting of stem cell colonies on microraft arrays. APL Bioeng 3:036106 (2019). PubMed: 31489396
- Li G et al. Generation and Characterization of Induced Pluripotent Stem Cells and Retinal Organoids From a Leber's Congenital Amaurosis Patient With Novel RPE65 Mutations. Front Mol Neurosci 12:212 (2019). PubMed: 31572124