Overview

  • Product name

    Anti-TRA-1-81 antibody [TRA-1-81]
  • Description

    Mouse monoclonal [TRA-1-81] to TRA-1-81
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IP, ICC, RIA, IHC-Frmore details
  • Species reactivity

    Reacts with: Rabbit, Human
    Does not react with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Human TRA-1-81.

  • Positive control

    • WB: HUES7 whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Purity

    Tissue culture supernatant
  • Purification notes

    Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • Clonality

    Monoclonal
  • Clone number

    TRA-1-81
  • Myeloma

    P3x66Ag.8-Sp2/0
  • Isotype

    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance

    The Podocalyxin gene encodes a member of the sialomucin protein family, and the encoded protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: playing a role in hematopoetic cell differentiation, binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements and being expressed in vascular endothelium cells and binding to L-selectin.
  • Cellular localization

    Membrane; Single-pass type I membrane protein
  • Database links

  • Alternative names

    • Gp200 antibody
    • PC antibody
    • PCLP antibody
    • PCLP1 antibody

Images

  • Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 200-400 kDa


    Exposure time: 20 minutes


    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • Anti-TRA-1-81 antibody [TRA-1-81] (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg

    Secondary
    Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 200-400 kDa


    Exposure time: 3 minutes
  • ab16289 at 1/100 staining human embryonic stem cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% goat serum prior to incubation with the antibody for 1 hour. An Alexa-Fluor 488 conjugated Goat anti-mouse IgM was used as the secondary antibody.

    DAPI staining is shown in the top panel while TRA-1-81 staining with ab16289 is shown in the lower panel.

    See Abreview

  • ab16289 staining TRA-1-81 from human embryonic stem cells by immunocytochemistry/immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in Triton prior to blocking in 10% serum for 1 hour at 24°C. The primary antibody was diluted 1/500 and incubated with the sample for 1 hour at 37°C. An Alexafluor 647 goat polyclonal to mouse IgM, diluted 1/200, was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Xu J  et al. Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system. Stem Cell Res Ther 10:193 (2019). Read more (PubMed: 31248457) »
  • Li G  et al. Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells Int 2018:4968658 (2018). Read more (PubMed: 30008752) »
See all 29 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Embryonic stem cell, HUES7)
Specification
Embryonic stem cell, HUES7
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Ms. Fiona Lewis

Verified customer

Submitted Oct 27 2009

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (embryonic stem cells)
Specification
embryonic stem cells
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%

Dr. Maria Keramari

Verified customer

Submitted Dec 06 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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