Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)
![Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)](https://www.abcam.com/ps/products/16/ab16289/Images/ab16289-276571-anti-tra-1-81-antibody-tra-1-81-western-blot.jpg "Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)")
Key features and details
- Mouse monoclonal [TRA-1-81] to TRA-1-81 - Stem Cell Marker
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker -
Description
Mouse monoclonal [TRA-1-81] to TRA-1-81 - Stem Cell Marker -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Tissue, cells or virus corresponding to Human TRA-1-81.
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Positive control
- WB: HUES7 whole cell lysate.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS -
Clonality
Monoclonal -
Clone number
TRA-1-81 -
Myeloma
P3x66Ag.8-Sp2/0 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| All applications |
Rabbit
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa.
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| Notes |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 200-400 kDa. |
Target
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Relevance
The Podocalyxin gene encodes a member of the sialomucin protein family, and the encoded protein was originally identified as an important component of glomerular podocytes. Other biological activities of the encoded protein include: playing a role in hematopoetic cell differentiation, binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements and being expressed in vascular endothelium cells and binding to L-selectin. -
Cellular localization
Membrane; Single-pass type I membrane protein -
Database links
- Entrez Gene: 5420 Human
- Omim: 602632 Human
- SwissProt: O00592 Human
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Alternative names
- Gp200 antibody
- PC antibody
- PCLP antibody
- PCLP1 antibody
Images
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Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + HUES7 (Human embryonic stem cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 200-400 kDa
Exposure time: 20 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16289 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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![Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)](https://www.abcam.com/ps/products/16/ab16289/Images/ab16289-24943-anti-tra-1-81-antibody-tra-1-81-western-blot.jpg)
Western blot - Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289)Anti-TRA-1-81 antibody [TRA-1-81] - Stem Cell Marker (ab16289) at 3 µg/ml + MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196) at 10 µg
Secondary
Goat F(ab')2 Anti-Mouse IgM mu chain (HRP) (ab5930) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 200-400 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (30)
ab16289 has been referenced in 30 publications.
- Xu J et al. Generation of pig induced pluripotent stem cells using an extended pluripotent stem cell culture system. Stem Cell Res Ther 10:193 (2019). PubMed: 31248457
- Li G et al. Generation of Retinal Organoids with Mature Rods and Cones from Urine-Derived Human Induced Pluripotent Stem Cells. Stem Cells Int 2018:4968658 (2018). PubMed: 30008752
- Li Y et al. Abnormal Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Partially Mimicked Development of TSC2 Neurological Abnormalities. Stem Cell Reports 8:883-893 (2017). IF ; Human . PubMed: 28344003
- Ding C et al. Different therapeutic effects of cells derived from human amniotic membrane on premature ovarian aging depend on distinct cellular biological characteristics. Stem Cell Res Ther 8:173 (2017). WB ; Human . PubMed: 28750654
- Eid W & Abdel-Rehim W Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A. Biol Chem N/A:N/A (2016). PubMed: 27343473