Product nameAnti-TRA2A antibody
See all TRA2A primary antibodies
DescriptionRabbit polyclonal to TRA2A
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab138448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Sequence similaritiesBelongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated in the RS domains.
- Information by UniProt
- AWMS1 antibody
- HSU53209 antibody
- htra-2 alpha antibody
All lanes : Anti-TRA2A antibody (ab138448) at 1/2000 dilution
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : 293T cell lysate at 50 µg
Lane 4 : Jurkat cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds
ab138448 at 6 μg/mg of lysate immunoprecipitating TRA2A in HeLa (human cervix adenocarcinoma) whole cell lysate.
Lane 1: ab137248 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
Lane 2: ab138448 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
Lane 3 (-): Control IgG + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)
For western blotting, ab138448 at 0.4 μg/mL
Detection: Chemiluminescence with exposure time of 10 seconds.
Immunoprecipitation detection of TRA2A in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138448 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138448 at 0.4µg/ml. Chemiluminescence with an exposure time of 30 seconds.
ab138448 has not yet been referenced specifically in any publications.