Overview

  • Product name

  • Description

    Rabbit polyclonal to TRA2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between amino acids 1 and 50 of Human TRA2A (NP_037425.1).

  • Positive control

    • HeLa, 293T and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab138448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 33 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated in the RS domains.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AWMS1 antibody
    • HSU53209 antibody
    • htra-2 alpha antibody
    • Putative MAPK activating protein PM24 antibody
    • TRA 2 alpha antibody
    • TRA 2A antibody
    • TRA-2 alpha antibody
    • TRA2 alpha antibody
    • TRA2-alpha antibody
    • TRA2A antibody
    • TRA2A_HUMAN antibody
    • Tra2alpha antibody
    • Transformer 2 alpha antibody
    • Transformer 2 protein homolog antibody
    • Transformer-2 protein homolog A antibody
    • Transformer-2 protein homolog alpha antibody
    see all

Images

  • All lanes : Anti-TRA2A antibody (ab138448) at 1/2000 dilution

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : 293T cell lysate at 50 µg
    Lane 4 : Jurkat cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 33 kDa


    Exposure time: 30 seconds
  • ab138448 at 6 μg/mg of lysate immunoprecipitating TRA2A in HeLa (human cervix adenocarcinoma) whole cell lysate.

    Lane 1: ab137248 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)

    Lane 2: ab138448 + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)

    Lane 3 (-): Control IgG + HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane)

    For western blotting, ab138448 at 0.4 μg/mL

    Detection: Chemiluminescence with exposure time of 10 seconds.

  • Immunoprecipitation detection of TRA2A in HeLa whole cell lysate (1 mg/IP; 20% of IP loaded/lane) using ab138448 at 6µg/mg lysate. Subsequent Western blot detection step was performed using ab138448 at 0.4µg/ml. Chemiluminescence with an exposure time of 30 seconds.

References

ab138448 has not yet been referenced specifically in any publications.

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