Overview

  • Product name

  • Description

    Rabbit polyclonal to TRA2A
  • Host species

    Rabbit
  • Specificity

    This antibody detects endogenous levels of total TRA2A protein.
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    A synthesized peptide derived from an internal region of human TRA2A.

  • Positive control

    • Hela cells, human brain tissue sections and extracts from HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 27, 33, 44 kDa (predicted molecular weight: 33 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Phosphorylated in the RS domains.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AWMS1 antibody
    • HSU53209 antibody
    • htra-2 alpha antibody
    • Putative MAPK activating protein PM24 antibody
    • TRA 2 alpha antibody
    • TRA 2A antibody
    • TRA-2 alpha antibody
    • TRA2 alpha antibody
    • TRA2-alpha antibody
    • TRA2A antibody
    • TRA2A_HUMAN antibody
    • Tra2alpha antibody
    • Transformer 2 alpha antibody
    • Transformer 2 protein homolog antibody
    • Transformer-2 protein homolog A antibody
    • Transformer-2 protein homolog alpha antibody
    see all

Images

  • All lanes : Anti-TRA2A antibody (ab72625) at 1/250 dilution

    Lanes 1 & 3 & 5 : 293T whole cell lysate
    Lanes 2 & 4 & 6 : Mouse ES (129Sv) whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 27 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)


    Exposure time: 1 minute

    See Abreview

  • All lanes : Anti-TRA2A antibody (ab72625) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa
    Additional bands at: 27 kDa, 44 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab72625 at 1:50 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • Immunofluorescence analysis of Hela cells using ab72625 at a 1:500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References

This product has been referenced in:

  • Hartmann H  et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). Read more (PubMed: 30456350) »
  • Cid-Samper F  et al. An Integrative Study of Protein-RNA Condensates Identifies Scaffolding RNAs and Reveals Players in Fragile X-Associated Tremor/Ataxia Syndrome. Cell Rep 25:3422-3434.e7 (2018). Read more (PubMed: 30566867) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Specification
mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Nov 11 2013

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