Anti-TRA2B/SFRS10 antibody (ab31353)
Key features and details
- Rabbit polyclonal to TRA2B/SFRS10
- Suitable for: IHC-P, WB, ICC/IF, CLIP
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRA2B/SFRS10 antibody
See all TRA2B/SFRS10 primary antibodies -
Description
Rabbit polyclonal to TRA2B/SFRS10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IF, CLIPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
Although the predicted molecular mass of TRA2B/SFRS10 is ~33 kDa, the available literature describes TRA2B/SFRS10 running at ~40 kDa in SDS-PAGE |
ICC/IF |
Use a concentration of 1 µg/ml.
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CLIP |
Use at an assay dependent concentration. PubMed: 22194695
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). Although the predicted molecular mass of TRA2B/SFRS10 is ~33 kDa, the available literature describes TRA2B/SFRS10 running at ~40 kDa in SDS-PAGE |
ICC/IF
Use a concentration of 1 µg/ml. |
CLIP
Use at an assay dependent concentration. PubMed: 22194695 |
Target
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Function
Sequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing. -
Tissue specificity
Highest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395403 Chicken
- Entrez Gene: 615156 Cow
- Entrez Gene: 6434 Human
- Entrez Gene: 20462 Mouse
- Entrez Gene: 117259 Rat
- Omim: 602719 Human
- SwissProt: Q3ZBT6 Cow
- SwissProt: P62995 Human
see all -
Alternative names
- arginine/serine-rich 10 antibody
- Arginine/serine-rich splicing factor 10 antibody
- hTRA2-beta antibody
see all
Images
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All lanes : Anti-TRA2B/SFRS10 antibody (ab31353) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutesAlthough the predicted molecular mass of TRA2B/SFRS10 is ~33 kDa, the available literature describes TRA2B/SFRS10 running at ~40 kDa in SDS-PAGE.
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ab31353 staining TRA2B in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab31353 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-TRA2B/SFRS10 antibody (ab31353) at 1/100 dilution + NSC34 mouse cell lysate at 20 µg
Secondary
IRDYE® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab31353 has been referenced in 25 publications.
- Pengelly RJ et al. Exonic splicing code and protein binding sites for calcium. Nucleic Acids Res 50:5493-5512 (2022). PubMed: 35474482
- Dahal S et al. The Thiazole-5-Carboxamide GPS491 Inhibits HIV-1, Adenovirus, and Coronavirus Replication by Altering RNA Processing/Accumulation. Viruses 14:N/A (2021). PubMed: 35062264
- White LA et al. Tra2beta-Dependent Regulation of RIO Kinase 3 Splicing During Rift Valley Fever Virus Infection Underscores the Links Between Alternative Splicing and Innate Antiviral Immunity. Front Cell Infect Microbiol 11:799024 (2021). PubMed: 35127560
- Wong RW et al. An activator of G protein-coupled receptor and MEK1/2-ERK1/2 signaling inhibits HIV-1 replication by altering viral RNA processing. PLoS Pathog 16:e1008307 (2020). PubMed: 32069328
- Oh J et al. Opposite Roles of Tra2ß and SRSF9 in the v10 Exon Splicing of CD44. Cancers (Basel) 12:N/A (2020). PubMed: 33143085