Product nameAnti-TRA2B antibody
See all TRA2B primary antibodies
DescriptionRabbit polyclonal to TRA2B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
- 293T Cell Lysate (for WB applications) and Skeletal muscle cells (for Immunohistochemistry)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionSequence-specific RNA-binding protein which participates in the control of pre-mRNA splicing.
Tissue specificityHighest expression in heart, skeletal muscle and pancreas. Less abundant in kidney, placenta and brain. Lowest expression in kidney and liver.
Sequence similaritiesBelongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated in the RS domains.
Dimethylation at Arg-241 is probably asymmetric.
- Information by UniProt
- arginine/serine-rich 10 antibody
- Arginine/serine-rich splicing factor 10 antibody
- hTRA2-beta antibody
Anti-TRA2B antibody (ab50846) at 5 µg/ml (in 5% skim milk / PBS buffer) + 239T Cell Lysate at 10 µg
HRP conjugated anti-Rabbit IgG should be diluted in 1/ 50,000 - 100,000
Predicted band size: 32 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Gel Concentration: 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling TRA2B with ab50846 at 4-8µg/ml. Arrows indicate positively stained human skeletal muscle cells. Magnification: 400X.
This product has been referenced in:
- Erkelenz S et al. Tra2-mediated recognition of HIV-1 5' splice site D3 as a key factor in the processing of vpr mRNA. J Virol 87:2721-34 (2013). Read more (PubMed: 23255807) »
- Uren PJ et al. Site identification in high-throughput RNA-protein interaction data. Bioinformatics 28:3013-20 (2012). IP . Read more (PubMed: 23024010) »