Overview

  • Product name

  • Description

    Rabbit polyclonal to TRADD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human TRADD aa 1-312.
    Database link: Q15628

  • Positive control

    • WB: K562 cell lysate; Rat kidney tissue lysate. IHC-P: Human liver and tonsil tissues. ICC/IF: U251 cells. IP: K562 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab223040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/200 - 1/2000.
WB 1/500 - 1/5000. Predicted molecular weight: 28,35 kDa.
IHC-P 1/20 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
  • Tissue specificity

    Found in all examined tissues.
  • Sequence similarities

    Contains 1 death domain.
  • Domain

    Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9130005N23Rik antibody
    • AA930854 antibody
    • TNFR1 associated DEATH domain protein antibody
    • TNFR1-associated DEATH domain protein antibody
    • TNFRSF1A associated via death domain antibody
    • TNFRSF1A-associated via death domain antibody
    • tradd antibody
    • TRADD_HUMAN antibody
    • Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody
    • Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
    see all

Images

  • All lanes : Anti-TRADD antibody (ab223040) at 1/500 dilution

    Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : Rat kidney tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 28,35 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human liver tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human tonsil tissue stained for TRADD using ab223040 at 1/100 dilution in immunohistochemical analysis.

  • U251 cells stained for TRADD (green) using ab223040 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • TRADD was immunoprecipitated from 0.5 mg of K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate with ab223040 at 1/200 dilution. Western blot was performed from the immunoprecipitate using ab223040 at 1/500 dilution. HRP-conjugated Protein G antibody was used as secondary antibody at 1/2000 dilution.

    Lane 1: Rabbit control IgG instead of ab223040 in K562 whole cell lysate.

    Lane 2: ab223040 IP in K562 whole cell lysate. 

    Lane 3: K562 whole cell lysate 10 µg (Input). 

     

References

ab223040 has not yet been referenced specifically in any publications.

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