Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3604] to TRADD
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-TRADD antibody [EPR3604]
See all TRADD primary antibodies
DescriptionRabbit monoclonal [EPR3604] to TRADD
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human TRADD aa 250-350. The exact sequence is proprietary.
- WB: Jurkat, MCF7, HeLa and K562 cell lysates, Human liver, and Human fetal spleen lysates. IHC-P: human breast carcinoma tissue. ICC/IF: MCF7 and HeLa cells. Flow Cyt: HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab110644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
For Mouse this antibody is recommended for WB only.
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol.
For unpurified use at 1/600
FunctionAdapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
Tissue specificityFound in all examined tissues.
Sequence similaritiesContains 1 death domain.
DomainRequires the intact DEATH domain to associate with TNFRSF1A/TNFR1.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 9130005N23Rik antibody
- AA930854 antibody
- TNFR1 associated DEATH domain protein antibody
All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : Human liver lysates
Lane 3 : Humanf fetal spleen lysates
Lysates/proteins at 15 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Immunocytochemistry/ Immunofluorescence analysis of MCF7(Human breast adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/100 dilution (6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TRADD with purified ab110644 at 1/850 dilution (0.7 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/600 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Unpurified ab110644, at 1/100, staining TRADD in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/500 dilution ((unpurified))
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Unpurified ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.
ab110644 has been referenced in 2 publications.
- Zhou J et al. Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. World J Gastroenterol 25:2071-2085 (2019). PubMed: 31114134
- Schmid H et al. Loss of inner retinal neurons after retinal ischemia in rats. Invest Ophthalmol Vis Sci 55:2777-87 (2014). PubMed: 24699380