Overview

  • Product name
    Anti-TRADD antibody [EPR3604]
    See all TRADD primary antibodies
  • Description
    Rabbit monoclonal [EPR3604] to TRADD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TRADD aa 250-350. The exact sequence is proprietary.

  • Positive control
    • Jurkat, MCF7, HeLa and K562 cell lysates Human breast carcinoma tissue HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab110644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IP 1/10.
IHC-P 1/100. Perform antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function
      Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
    • Tissue specificity
      Found in all examined tissues.
    • Sequence similarities
      Contains 1 death domain.
    • Domain
      Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
      • Alternative names
        • 9130005N23Rik antibody
        • AA930854 antibody
        • TNFR1 associated DEATH domain protein antibody
        • TNFR1-associated DEATH domain protein antibody
        • TNFRSF1A associated via death domain antibody
        • TNFRSF1A-associated via death domain antibody
        • tradd antibody
        • TRADD_HUMAN antibody
        • Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody
        • Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
        see all

      Images

      • Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
        Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
        All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/500 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : MCF7 cell lysate
        Lane 3 : HeLa cell lysate
        Lane 4 : K562 cell lysate

        Secondary
        All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

        Predicted band size: 34 kDa
        Observed band size: 34 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
        ab110644, at 1/100, staining TRADD in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
      • Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
        Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
        ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.

      References

      This product has been referenced in:

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