Recombinant Anti-TRADD antibody [EPR3604] (ab110644)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3604] to TRADD
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TRADD antibody [EPR3604]
See all TRADD primary antibodies -
Description
Rabbit monoclonal [EPR3604] to TRADD -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat, MCF7, HeLa and K562 cell lysates, Human liver, and Human fetal spleen lysates. IHC-P: human breast carcinoma tissue. ICC/IF: MCF7 and HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3604 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/100.
|
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Flow Cyt (Intra) |
1/30.
For unpurified use at 1/600 |
|
WB |
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
For Mouse this antibody is recommended for WB only. |
|
IHC-P |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol. |
Notes |
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ICC/IF
1/50 - 1/100. |
Flow Cyt (Intra)
1/30. For unpurified use at 1/600 |
WB
1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). For Mouse this antibody is recommended for WB only. |
IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. -
Tissue specificity
Found in all examined tissues. -
Sequence similarities
Contains 1 death domain. -
Domain
Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 8717 Human
- Omim: 603500 Human
- SwissProt: Q15628 Human
- Unigene: 460996 Human
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Alternative names
- 9130005N23Rik antibody
- AA930854 antibody
- TNFR1 associated DEATH domain protein antibody
see all
Images
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All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : Human liver lysates
Lane 3 : Humanf fetal spleen lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
Immunocytochemistry/ Immunofluorescence analysis of MCF7(Human breast adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/100 dilution (6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TRADD with purified ab110644 at 1/850 dilution (0.7 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/600 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
Unpurified ab110644, at 1/100, staining TRADD in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/500 dilution ((unpurified))
Lane 1 : Jurkat cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa -
Unpurified ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab110644 has been referenced in 7 publications.
- Roedig J et al. USP22 controls necroptosis by regulating receptor-interacting protein kinase 3 ubiquitination. EMBO Rep 22:e50163 (2021). PubMed: 33369872
- Lu C et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
- Ling P et al. miR-1184 regulates inflammatory responses and cell apoptosis by targeting TRADD in an LPS-induced cell model of sepsis. Exp Ther Med 21:630 (2021). PubMed: 33936286
- Liu H et al. Copper Induces Oxidative Stress and Apoptosis in the Mouse Liver. Oxid Med Cell Longev 2020:1359164 (2020). PubMed: 32411316
- Wang X et al. Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis. Mediators Inflamm 2019:2945083 (2019). PubMed: 31885495
- Zhou J et al. Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. World J Gastroenterol 25:2071-2085 (2019). PubMed: 31114134
- Schmid H et al. Loss of inner retinal neurons after retinal ischemia in rats. Invest Ophthalmol Vis Sci 55:2777-87 (2014). PubMed: 24699380