Recombinant
RabMAb

Recombinant Anti-TRADD antibody [EPR3604] (ab110644)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3604] to TRADD
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TRADD antibody [EPR3604]
    See all TRADD primary antibodies

  • Description

    Rabbit monoclonal [EPR3604] to TRADD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human TRADD aa 250-350. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, MCF7, HeLa and K562 cell lysates, Human liver, and Human fetal spleen lysates. IHC-P: human breast carcinoma tissue. ICC/IF: MCF7 and HeLa cells. Flow Cyt: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab110644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

For Mouse this antibody is recommended for WB only.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Flow Cyt 1/30.

For unpurified use at 1/600

Target

  • Function

    Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.

  • Tissue specificity

    Found in all examined tissues.

  • Sequence similarities

    Contains 1 death domain.

  • Domain

    Requires the intact DEATH domain to associate with TNFRSF1A/TNFR1.

  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • Database links

    • Alternative names

      • 9130005N23Rik antibody
      • AA930854 antibody
      • TNFR1 associated DEATH domain protein antibody
      • TNFR1-associated DEATH domain protein antibody
      • TNFRSF1A associated via death domain antibody
      • TNFRSF1A-associated via death domain antibody
      • tradd antibody
      • TRADD_HUMAN antibody
      • Tumor necrosis factor receptor type 1 associated DEATH domain protein antibody
      • Tumor necrosis factor receptor type 1-associated DEATH domain protein antibody
      see all

    Images

    • Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
      Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
      All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/1000 dilution (Purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
      Lane 2 : Human liver lysates
      Lane 3 : Humanf fetal spleen lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
      Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
      Immunocytochemistry/ Immunofluorescence analysis of MCF7(Human breast adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/100 dilution (6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling TRADD with purified ab110644 at 1/850 dilution (0.7 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Flow Cytometry - Anti-TRADD antibody [EPR3604] (ab110644)
      Flow Cytometry - Anti-TRADD antibody [EPR3604] (ab110644)
      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRADD with purified ab110644 at 1/600 dilution (1 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRADD antibody [EPR3604] (ab110644)

      Unpurified ab110644, at 1/100, staining TRADD in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
      Western blot - Anti-TRADD antibody [EPR3604] (ab110644)
      All lanes : Anti-TRADD antibody [EPR3604] (ab110644) at 1/500 dilution ((unpurified))

      Lane 1 : Jurkat cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)
      Immunocytochemistry/ Immunofluorescence - Anti-TRADD antibody [EPR3604] (ab110644)

      Unpurified ab110644, at 1/100, staining TRADD in HeLa cells by Immunofluorescence.

    References (2)

    Publishing research using ab110644? Please let us know so that we can cite the reference in this datasheet.

    ab110644 has been referenced in 2 publications.

    • Zhou J  et al. Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. World J Gastroenterol 25:2071-2085 (2019). PubMed: 31114134
    • Schmid H  et al. Loss of inner retinal neurons after retinal ischemia in rats. Invest Ophthalmol Vis Sci 55:2777-87 (2014). PubMed: 24699380

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