Anti-TRAF1 antibody (ab129279)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-TRAF1 antibody
See all TRAF1 primary antibodies -
Description
Goat polyclonal to TRAF1 -
Host species
Goat -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken, Cow, Dog, Zebrafish, a wide range of other species
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Immunogen
Synthetic peptide:
KLQSPKHAYVKDD
, corresponding to C terminal amino acids 392-404 of Human TRAF1 (NP_005649.1). -
Positive control
- Human Small Intestine and Thymus tissues; U937 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 24284943 | |
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 46 kDa. | |
| IHC-P | Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | 1/16000. Optimal dilution to be determined by the researcher |
Target
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Function
Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. -
Sequence similarities
Contains 1 MATH domain. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling. -
Post-translational
modificationsPolyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation. - Information by UniProt
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Database links
- Entrez Gene: 7185 Human
- Entrez Gene: 22029 Mouse
- Omim: 601711 Human
- SwissProt: Q13077 Human
- SwissProt: P39428 Mouse
- Unigene: 531251 Human
- Unigene: 239514 Mouse
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Alternative names
- 4732496E14Rik antibody
- EBI6 antibody
- EBV induced protein 6 antibody
see all
Images
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Western blot - Anti-TRAF1 antibody (ab129279)Anti-TRAF1 antibody (ab129279) at 0.5 µg/ml (for 1 hour) + U937 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (ab129279)ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human small intestine tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (ab129279)ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Lu YY et al. TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death. Nat Commun 4:2852 (2013). IHC-Fr ; Mouse . Read more (PubMed: 24284943) »
