Anti-TRAF1 antibody (ab129279)
Key features and details
- Goat polyclonal to TRAF1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TRAF1 antibody
See all TRAF1 primary antibodies -
Description
Goat polyclonal to TRAF1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Chicken, Cow, Dog, Zebrafish, a wide range of other species
-
Immunogen
Synthetic peptide:
KLQSPKHAYVKDD
, corresponding to C terminal amino acids 392-404 of Human TRAF1 (NP_005649.1). -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab129279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 46 kDa. | |
| IHC-P | Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2. -
Sequence similarities
Contains 1 MATH domain. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
Cleavage by CASP8 liberates a C-terminal fragment that promotes apoptosis and inhibits the activation of NF-kappa-B in response to TNF signaling. -
Post-translational
modificationsPolyubiquitinated by BIRC2 and/or BIRC3, leading to its subsequent proteasomal degradation. - Information by UniProt
-
Database links
- Entrez Gene: 7185 Human
- Omim: 601711 Human
- SwissProt: Q13077 Human
- Unigene: 531251 Human
-
Alternative names
- 4732496E14Rik antibody
- EBI6 antibody
- EBV induced protein 6 antibody
see all
Images
-
Western blot - Anti-TRAF1 antibody (ab129279)Anti-TRAF1 antibody (ab129279) at 0.5 µg/ml (for 1 hour) + U937 cell lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (ab129279)ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human small intestine tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF1 antibody (ab129279)ab129279, at 3.75 µg/ml, staining TRAF1 in Formalin-fixed, Paraffin-embedded Human thymus tissue by Immunohistochemistry.
Protocols
Datasheets and documents
References (1)
ab129279 has been referenced in 1 publication.
- Lu YY et al. TRAF1 is a critical regulator of cerebral ischaemia-reperfusion injury and neuronal death. Nat Commun 4:2852 (2013). IHC-Fr ; Mouse . PubMed: 24284943
