Overview

  • Product name

  • Description

    Rabbit polyclonal to TRAF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human TRAF2 aa 300-350. The exact sequence is proprietary. (NP_066961.2).
    Database link: Q12933

  • Positive control

    • IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma and colon carconoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab225888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the TNF receptor-associated factor family. A subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 2 TRAF-type zinc fingers.
    • Domain

      The coiled coil domain mediates homo- and hetero-oligomerization.
      The MATH/TRAF domain binds to receptor cytoplasmic domains.
      The RING-type zinc finger domain is essential for E3 ubiquitin-protein ligase activity. It is not essential for the stabilization of BIRC2, or for the ubiquitination of RIPK1 in response to TNFR1 signaling.
    • Post-translational
      modifications

      Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.
      Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • E3 ubiquitin-protein ligase TRAF2 antibody
      • MGC:45012 antibody
      • OTTHUMP00000022625 antibody
      • OTTHUMP00000064745 antibody
      • TNF receptor associated factor 2 antibody
      • TNF receptor-associated factor 2 antibody
      • TNF receptor-associated protein antibody
      • TRAF 2 antibody
      • TRAF2 antibody
      • TRAF2_HUMAN antibody
      • TRAP 3 antibody
      • TRAP antibody
      • TRAP3 antibody
      • Tumor necrosis factor type 2 receptor associated protein 3 antibody
      • Tumor necrosis factor type 2 receptor-associated protein 3 antibody
      see all

    Images

    • TRAF2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225888 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a rabbit anti-TRAF2 antibody which recognizes an upstream epitope at 0.4 µg/ml.

      Lane 1: ab225888 IP in HeLa whole cell lysate.

      Lane 2: Control IgG IP in HeLa whole cell lysate.

      Detection: Chemiluminescence with exposure time of 10 seconds.

    • Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for TRAF2 using ab225888 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for TRAF2 using ab225888 at 1/5000 dilution in immunohistochemical analysis.

      Detection: DAB staining.

    References

    ab225888 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab225888.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up