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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-TRAF2 antibody (ab244314)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (ab244314)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody (ab244314)
  • Western blot - Anti-TRAF2 antibody (ab244314)
  • Western blot - Anti-TRAF2 antibody (ab244314)

Key features and details

  • Rabbit polyclonal to TRAF2
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-TRAF2 antibody
    See all TRAF2 primary antibodies
  • Description

    Rabbit polyclonal to TRAF2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human TRAF2 aa 200-350.
    Database link: Q12933

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: RT4 and U-251 MG cell lysates; TRAF2 overexpression HEK-293T cell lysate. IHC-P: Human testis tissue. ICC/IF: U-251 MG cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Signal transduction
    • Other
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Recombinant Protein

    • Recombinant Human TRAF2 protein (ab204091)
  • Related Products

    • Recombinant Human TRAF2 protein (ab204091)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab244314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    The RING-type zinc finger domain is essential for E3 ubiquitin-protein ligase activity. It is not essential for the stabilization of BIRC2, or for the ubiquitination of RIPK1 in response to TNFR1 signaling.
  • Post-translational
    modifications

    Phosphorylated at several serine residues within the first 128 amino acid residues. Phosphorylated at Thr-117 in response to signaling via TNF and TNFRSF1A. Phosphorylation at Thr-117 is required for 'Lys-63'-linked polyubiquitination, but not for 'Lys-48'-linked polyubiquitination. Phosphorylation at Thr-117 is important for interaction with IKKA and IKKB, activation of IKK and subsequent activation of NF-kappa-B.
    Undergoes both 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination. Polyubiquitinated via 'Lys-63'-linked ubiquitin in response to TNF signaling; this requires prior phosphorylation at Thr-117. 'Lys-63'-linked polyubiquitination promotes TRAF2-mediated activation of NF-kappa-B. Can be polyubiquitinated at several Lys residues via 'Lys-48'-linked ubiquitin chains in response to TNF signaling, leading to proteasomal degradation. Autoubiquitinated, leading to its subsequent proteasomal degradation. Polyubiquitinated by BIRC2 and SIAH2, leading to its subsequent proteasomal degradation. Deubiquitinated by CYLD, a protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q12933 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7186 Human
    • Omim: 601895 Human
    • SwissProt: Q12933 Human
    • Unigene: 522506 Human
    • Alternative names

      • E3 ubiquitin-protein ligase TRAF2 antibody
      • MGC:45012 antibody
      • OTTHUMP00000022625 antibody
      • OTTHUMP00000064745 antibody
      • TNF receptor associated factor 2 antibody
      • TNF receptor-associated factor 2 antibody
      • TNF receptor-associated protein antibody
      • TRAF 2 antibody
      • TRAF2 antibody
      • TRAF2_HUMAN antibody
      • TRAP 3 antibody
      • TRAP antibody
      • TRAP3 antibody
      • Tumor necrosis factor type 2 receptor associated protein 3 antibody
      • Tumor necrosis factor type 2 receptor-associated protein 3 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (ab244314)
      Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody (ab244314)

      PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for TRAF2 (green) using ab244314 at 4 µg/ml in ICC/IF.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody (ab244314)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody (ab244314)

      Formalin-fixed, paraffin-embedded human testis tissue stained for TRAF2 using ab244314 at 1/20 dilution in immunohistochemical analysis.

    • Western blot - Anti-TRAF2 antibody (ab244314)
      Western blot - Anti-TRAF2 antibody (ab244314)
      All lanes : Anti-TRAF2 antibody (ab244314) at 0.4 µg/ml

      Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
      Lane 2 : TRAF2 overexpression HEK-293T cell lysate

      Predicted band size: 56 kDa

    • Western blot - Anti-TRAF2 antibody (ab244314)
      Western blot - Anti-TRAF2 antibody (ab244314)
      All lanes : Anti-TRAF2 antibody (ab244314) at 0.4 µg/ml

      Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
      Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
      Lane 3 : Human plasma

      Predicted band size: 56 kDa

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab244314? Please let us know so that we can cite the reference in this datasheet.

    ab244314 has not yet been referenced specifically in any publications.

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