Anti-TRAF3 antibody (ab155298)
Key features and details
- Rabbit polyclonal to TRAF3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAF3 antibody
See all TRAF3 primary antibodies -
Description
Rabbit polyclonal to TRAF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 61-300 of Human TRAF3 (Uniprot: Q13114)
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Positive control
- 293T; H1299; HeLa; HepG2 or Raji cell lysates, Hela cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. -
Sequence similarities
Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. -
Domain
The MATH/TRAF domain binds to receptor cytoplasmic domains.
The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing. -
Post-translational
modificationsUndergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5. -
Cellular localization
Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus. - Information by UniProt
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Database links
- Entrez Gene: 7187 Human
- Entrez Gene: 22031 Mouse
- Omim: 601896 Human
- SwissProt: Q13114 Human
- SwissProt: Q60803 Mouse
- Unigene: 510528 Human
- Unigene: 27431 Mouse
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Alternative names
- CAP 1 antibody
- CAP-1 antibody
- CAP1 antibody
see all
Images
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Anti-TRAF3 antibody (ab155298) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 64 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling TRAF3 with ab155298 at 1/200. Lower image shows costaining with a DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab155298 has been referenced in 1 publication.
- Bustos BI et al. Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry. Sci Rep 9:772 (2019). PubMed: 30692554