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  1. Link

    traf3-antibody-ab155298.pdf

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Immunology Innate Immunity Cytokines TNF Superfamily
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Anti-TRAF3 antibody (ab155298)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Anti-TRAF3 antibody (ab155298)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAF3 antibody (ab155298)

Key features and details

  • Rabbit polyclonal to TRAF3
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human TRAF3 protein (ab132121)
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Overview

  • Product name

    Anti-TRAF3 antibody
    See all TRAF3 primary antibodies
  • Description

    Rabbit polyclonal to TRAF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 61-300 of Human TRAF3 (Uniprot: Q13114)

  • Positive control

    • 293T; H1299; HeLa; HepG2 or Raji cell lysates, Hela cells
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • TRAF
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • TRAF

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant Human TRAF3 protein (ab132121)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Detects a band of approximately 62 kDa (predicted molecular weight: 64 kDa).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • Domain

    The MATH/TRAF domain binds to receptor cytoplasmic domains.
    The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing.
  • Post-translational
    modifications

    Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.
  • Cellular localization

    Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
  • Target information above from: UniProt accession Q13114 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7187 Human
    • Entrez Gene: 22031 Mouse
    • Omim: 601896 Human
    • SwissProt: Q13114 Human
    • SwissProt: Q60803 Mouse
    • Unigene: 510528 Human
    • Unigene: 27431 Mouse
    • Alternative names

      • CAP 1 antibody
      • CAP-1 antibody
      • CAP1 antibody
      • CD40 associated protein 1 antibody
      • CD40 binding protein antibody
      • CD40 bp antibody
      • CD40 receptor associated factor 1 antibody
      • CD40 receptor-associated factor 1 antibody
      • CD40-binding protein antibody
      • CD40BP antibody
      • CRAF 1 antibody
      • CRAF1 antibody
      • IIAE5 antibody
      • LAP 1 antibody
      • LAP1 antibody
      • LMP1 associated protein antibody
      • LMP1-associated protein 1 antibody
      • MIPT3 antibody
      • TNF receptor associated factor 3 antibody
      • TNF receptor-associated factor 3 antibody
      • TRAF 3 antibody
      • Traf3 antibody
      • TRAF3_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TRAF3 antibody (ab155298)
      Western blot - Anti-TRAF3 antibody (ab155298)
      Anti-TRAF3 antibody (ab155298) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

      Predicted band size: 64 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-TRAF3 antibody (ab155298)
      Immunocytochemistry/ Immunofluorescence - Anti-TRAF3 antibody (ab155298)
      Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling TRAF3 with ab155298 at 1/200. Lower image shows costaining with a DNA probe.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab155298? Please let us know so that we can cite the reference in this datasheet.

    ab155298 has been referenced in 1 publication.

    • Bustos BI  et al. Variants in ABCG8 and TRAF3 genes confer risk for gallstone disease in admixed Latinos with Mapuche Native American ancestry. Sci Rep 9:772 (2019). PubMed: 30692554

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