Overview

  • Product name

    Anti-TRAF3 antibody [EPR22992-93]
    See all TRAF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR22992-93] to TRAF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human TRAF3 aa 300-400. The exact sequence is proprietary.
    Database link: Q13114

  • Positive control

    • WB: Wild type HAP1 whole cell lysate. Daudi, HEK-293T, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysate. Mouse brain, rat spleen and rat brain lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 64 kDa.

 

 

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.
    • Sequence similarities

      Belongs to the TNF receptor-associated factor family. A subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 2 TRAF-type zinc fingers.
    • Domain

      The MATH/TRAF domain binds to receptor cytoplasmic domains.
      The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing.
    • Post-translational
      modifications

      Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.
    • Cellular localization

      Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAP 1 antibody
      • CAP-1 antibody
      • CAP1 antibody
      • CD40 associated protein 1 antibody
      • CD40 binding protein antibody
      • CD40 bp antibody
      • CD40 receptor associated factor 1 antibody
      • CD40 receptor-associated factor 1 antibody
      • CD40-binding protein antibody
      • CD40BP antibody
      • CRAF 1 antibody
      • CRAF1 antibody
      • IIAE5 antibody
      • LAP 1 antibody
      • LAP1 antibody
      • LMP1 associated protein antibody
      • LMP1-associated protein 1 antibody
      • MIPT3 antibody
      • TNF receptor associated factor 3 antibody
      • TNF receptor-associated factor 3 antibody
      • TRAF 3 antibody
      • Traf3 antibody
      • TRAF3_HUMAN antibody
      see all

    Images

    • All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution

      Lane 1 : Wild type HAP1 whole cell lysate
      Lane 2 : TRAF3 knockout HAP1 whole cell lysate
      Lane 3 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
      Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      ab239357 was shown to specifically react with TRAF3 in wild-type HAP1 cells as signal was lost in TRAF3 knockout cells. Wild-type and TRAF3 knockout samples were subjected to SDS-PAGE. ab239357 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging.  Exposure time: 37 seconds.

    • All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution

      Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 5 : Mouse brain tissue lysate
      Lane 6 : Rat spleen tissue lysate
      Lane 7 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: Lanes 1-4: 128 seconds; Lane 5: 3 minutes;  Lane 6: 125 seconds; Lane 7: 8 seconds.

    References

    ab239357 has not yet been referenced specifically in any publications.

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