Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TRAF3 antibody [EPR22992-93] (ab239357)

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Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
  • Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
  • Anti-TRAF3 antibody [EPR22992-93] (ab239357)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22992-93] to TRAF3
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TRAF3 antibody [EPR22992-93]
    See all TRAF3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22992-93] to TRAF3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human TRAF3 aa 300-400. The exact sequence is proprietary.
    Database link: Q13114

  • Positive control

    • WB: Wild type HAP1 whole cell lysate. Daudi, HEK-293T, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysate. Mouse brain, rat spleen and rat brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab239357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 64 kDa.

 

 
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Regulates pathways leading to the activation of NF-kappa-B and MAP kinases, and plays a central role in the regulation of B cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa-B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14.

    • Sequence similarities

      Belongs to the TNF receptor-associated factor family. A subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 2 TRAF-type zinc fingers.

    • Domain

      The MATH/TRAF domain binds to receptor cytoplasmic domains.
      The Ring-type zinc finger domain is required for its function in down-regulation of NFKB2 proteolytic processing.

    • Post-translational
      modifications

      Undergoes 'Lys-48'-linked polyubiquitination, leading to its proteasomal degradation in response to signaling by TNFSF13B, TLR4 or through CD40. Undergoes 'Lys-63'-linked ubiquitination during early stages of virus infection, and 'Lys-48'-linked ubiquitination during later stages. Undergoes both 'Lys-48'-linked and 'Lys-63'-linked ubiquitination in response to TLR3 and TLR4 signaling (By similarity). Deubiquitinated by OTUB1, OTUB2 and OTUD5.

    • Cellular localization

      Cytoplasm. Endosome. Mitochondrion. Undergoes endocytosis together with TLR4 upon LPS signaling (By similarity). Associated with mitochondria in response to virus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CAP 1 antibody
      • CAP-1 antibody
      • CAP1 antibody
      • CD40 associated protein 1 antibody
      • CD40 binding protein antibody
      • CD40 bp antibody
      • CD40 receptor associated factor 1 antibody
      • CD40 receptor-associated factor 1 antibody
      • CD40-binding protein antibody
      • CD40BP antibody
      • CRAF 1 antibody
      • CRAF1 antibody
      • IIAE5 antibody
      • LAP 1 antibody
      • LAP1 antibody
      • LMP1 associated protein antibody
      • LMP1-associated protein 1 antibody
      • MIPT3 antibody
      • TNF receptor associated factor 3 antibody
      • TNF receptor-associated factor 3 antibody
      • TRAF 3 antibody
      • Traf3 antibody
      • TRAF3_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
      Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
      All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution

      Lane 1 : Wild type HAP1 whole cell lysate
      Lane 2 : TRAF3 knockout HAP1 whole cell lysate
      Lane 3 : Daudi (human Burkitts lymphoma lymphoblast), whole cell lysate
      Lane 4 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      ab239357 was shown to specifically react with TRAF3 in wild-type HAP1 cells as signal was lost in TRAF3 knockout cells. Wild-type and TRAF3 knockout samples were subjected to SDS-PAGE. ab239357 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging.  Exposure time: 37 seconds.

    • Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
      Western blot - Anti-TRAF3 antibody [EPR22992-93] (ab239357)
      All lanes : Anti-TRAF3 antibody [EPR22992-93] (ab239357) at 1/1000 dilution

      Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
      Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
      Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
      Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
      Lane 5 : Mouse brain tissue lysate
      Lane 6 : Rat spleen tissue lysate
      Lane 7 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 64 kDa
      Observed band size: 64 kDa



      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Exposure time: Lanes 1-4: 128 seconds; Lane 5: 3 minutes;  Lane 6: 125 seconds; Lane 7: 8 seconds.

    • Anti-TRAF3 antibody [EPR22992-93] (ab239357)
      Anti-TRAF3 antibody [EPR22992-93] (ab239357)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab239357? Please let us know so that we can cite the reference in this datasheet.

    ab239357 has not yet been referenced specifically in any publications.

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