Overview

  • Product name

  • Description

    Rabbit polyclonal to TRAF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TRAF4 aa 225-275. The exact sequence is proprietary. NP_004286.2
    Database link: Q9BUZ4

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab245666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.

Target

  • Function

    Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
  • Tissue specificity

    Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. B subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 3 TRAF-type zinc fingers.
  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Post-translational
    modifications

    Polyubiquitinated, leading to its proteasomal degradation.
  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • CART 1 antibody
    • CART1 antibody
    • cysteine rich domain associated with ring and TRAF domain antibody
    • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
    • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
    • Malignant 62 antibody
    • Metastatic lymph node gene 62 protein antibody
    • MLN 62 antibody
    • MLN62 antibody
    • RING finger protein 83 antibody
    • RNF 83 antibody
    • RNF83 antibody
    • TNF receptor associated factor 4 antibody
    • TNF receptor-associated factor 4 antibody
    • TRAF 4 antibody
    • Traf4 antibody
    • TRAF4_HUMAN antibody
    • tumor necrosis receptor-associated factor 4A antibody
    see all

Images

  • All lanes : Anti-TRAF4 antibody (ab245666) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 54 kDa


    Exposure time: 30 seconds


    Prepared using NETN lysis buffer.

  • TRAF4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

    ab245666 used for IP at 3 µg per reaction. For WB 1 µg/ml.

    Lane 1: ab245666 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Chemiluminescence detection: 30 seconds.

References

ab245666 has not yet been referenced specifically in any publications.

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