Product nameAnti-TRAF4 antibody
See all TRAF4 primary antibodies
DescriptionRabbit polyclonal to TRAF4
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human TRAF4 aa 420-470. The exact sequence is proprietary. NP_004286.2
Database link: Q9BUZ4
- IHC-P: Human lung cancer tissue. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245667 was affinity purified using an epitope specific to TRAF4 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAdapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
Tissue specificityExpressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
Sequence similaritiesBelongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers.
DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
TRAF4 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245667 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245667 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for TRAF4 with ab245667 at a 1/1000 dilution in immunohistochemical analysis. DAB staining.
ab245667 has not yet been referenced specifically in any publications.