Key features and details
- Rabbit polyclonal to TRAF4 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-TRAF4 antibody - C-terminal
See all TRAF4 primary antibodies
DescriptionRabbit polyclonal to TRAF4 - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat thymus, Hela, Jurkat, Hepa lysates. Rat lymphonodus tissue and human lung cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 54 kDa.|
FunctionAdapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
Tissue specificityExpressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
Sequence similaritiesBelongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers.
DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
All lanes : Anti-TRAF4 antibody - C-terminal (ab190986) at 0.5 µg/ml
Lane 1 : Rat thymus lysate
Lane 2 : HeLa lysate
Lane 3 : Jurkat lysate
Lane 4 : Hepa lysate
Predicted band size: 54 kDa
Immunohistochemical analysis of formalin fixed paraffin embedded human lung cancer tissue labeling TRAF4 with ab190986 at 1 µg/ml.
Immunohistochemical analysis of formalin fixed paraffin embedded rat lymphonodus tissue labeling TRAF4 with ab190986 at 1 µg/ml.
ab190986 has been referenced in 2 publications.
- Kang Q et al. Role of the overexpression of TRAF4 in predicting the prognosis of intrahepatic cholangiocarcinoma. Int J Oncol 53:286-296 (2018). PubMed: 29749456
- Meerson A & Yehuda H Leptin and insulin up-regulate miR-4443 to suppress NCOA1 and TRAF4, and decrease the invasiveness of human colon cancer cells. BMC Cancer 16:882 (2016). WB ; Human . PubMed: 27842582