Product nameAnti-TRAF4 antibody [EPR1729]
See all TRAF4 primary antibodies
DescriptionRabbit monoclonal [EPR1729] to TRAF4
Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human TRAF4 aa 350-450 (internal sequence). The exact sequence is proprietary.
- Jurkat, HeLa and SKBR-3 cell lysates
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
Tissue specificityExpressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
Sequence similaritiesBelongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers.
DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation.
Cellular localizationCytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
- Information by UniProt
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa