• Product name

    Anti-TRAF4 antibody [EPR1729]
    See all TRAF4 primary antibodies
  • Description

    Rabbit monoclonal [EPR1729] to TRAF4
  • Host species

  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human TRAF4 aa 350-450 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • Jurkat, HeLa and SKBR-3 cell lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions.
    • Tissue specificity

      Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus.
    • Sequence similarities

      Belongs to the TNF receptor-associated factor family. B subfamily.
      Contains 1 MATH domain.
      Contains 1 RING-type zinc finger.
      Contains 3 TRAF-type zinc fingers.
    • Domain

      The coiled coil domain mediates homo- and hetero-oligomerization.
      The MATH/TRAF domain binds to receptor cytoplasmic domains.
    • Post-translational

      Polyubiquitinated, leading to its proteasomal degradation.
    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links

    • Alternative names

      • CART 1 antibody
      • CART1 antibody
      • cysteine rich domain associated with ring and TRAF domain antibody
      • Cysteine rich domain associated with RING and Traf domains protein 1 antibody
      • Cysteine-rich domain associated with RING and Traf domains protein 1 antibody
      • Malignant 62 antibody
      • Metastatic lymph node gene 62 protein antibody
      • MLN 62 antibody
      • MLN62 antibody
      • RING finger protein 83 antibody
      • RNF 83 antibody
      • RNF83 antibody
      • TNF receptor associated factor 4 antibody
      • TNF receptor-associated factor 4 antibody
      • TRAF 4 antibody
      • Traf4 antibody
      • TRAF4_HUMAN antibody
      • tumor necrosis receptor-associated factor 4A antibody
      see all


    • Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa


    This product has been referenced in:

    • Zepp JA  et al. Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes. J Immunol 189:33-7 (2012). Read more (PubMed: 22649194) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Western blot
    Human Cell lysate - whole cell (LnCaP cells)
    Loading amount
    50 µg
    LnCaP cells
    Gel Running Conditions
    Reduced Denaturing (5-15% gradient gel)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

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    Submitted Nov 14 2012

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