Recombinant Anti-TRAF4 antibody [EPR1729] (ab108991)
![Flow Cytometry - Anti-TRAF4 antibody [EPR1729] (ab108991)](https://www.abcam.com/ps/products/108/ab108991/Images/ab108991-195332-anti-traf4-antibody-epr1729-flow-cytometry.jpg "Flow Cytometry - Anti-TRAF4 antibody [EPR1729] (ab108991)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1729] to TRAF4
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-TRAF4 antibody [EPR1729]
See all TRAF4 primary antibodies -
Description
Rabbit monoclonal [EPR1729] to TRAF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human TRAF4 aa 350-450 (internal sequence). The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa and SKBR-3 cell lysates
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1729 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 54 kDa. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract (By similarity). Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. -
Tissue specificity
Expressed in epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus. -
Sequence similarities
Belongs to the TNF receptor-associated factor family. B subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 3 TRAF-type zinc fingers. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Cell junction > tight junction. Cell membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 9618 Human
- Entrez Gene: 22032 Mouse
- Omim: 602464 Human
- SwissProt: Q9BUZ4 Human
- SwissProt: Q61382 Mouse
- Unigene: 8375 Human
- Unigene: 390418 Mouse
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Alternative names
- CART 1 antibody
- CART1 antibody
- cysteine rich domain associated with ring and TRAF domain antibody
see all
Images
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Flow Cytometry - Anti-TRAF4 antibody [EPR1729] (ab108991)Overlay histogram showing HeLa cells stained with ab108991 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108991, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-TRAF4 antibody [EPR1729] (ab108991)](https://www.abcam.com/ps/products/108/ab108991/Images/ab108991-121524-anti-traf4-antibody-epr1729-western-blot.jpg)
Western blot - Anti-TRAF4 antibody [EPR1729] (ab108991)All lanes : Anti-TRAF4 antibody [EPR1729] (ab108991) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa -
![Anti-TRAF4 antibody [EPR1729] (ab108991)](https://www.abcam.com/ps/products/108/ab108991/Images/ab108991-3-benefits-of-recombinant-antibodies.png)
Anti-TRAF4 antibody [EPR1729] (ab108991)
Protocols
Datasheets and documents
References (2)
ab108991 has been referenced in 2 publications.
- Wang J et al. Long non-coding RNA DLEU1 promotes cell proliferation of glioblastoma multiforme. Mol Med Rep 20:1873-1882 (2019). PubMed: 31257517
- Zepp JA et al. Cutting edge: TNF receptor-associated factor 4 restricts IL-17-mediated pathology and signaling processes. J Immunol 189:33-7 (2012). PubMed: 22649194
