Overview

  • Product name

  • Description

    Rabbit polyclonal to TRAF5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-186 of Human TRAF5 (IniProt ID: O00463).

  • Positive control

    • HeLa and Jurkat whole cell lysate (ab7899); HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis.
  • Tissue specificity

    Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • Domain

    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC:39780 antibody
    • RING finger protein 84 antibody
    • RNF 84 antibody
    • RNF84 antibody
    • TNF receptor associated factor 5 antibody
    • TNF receptor-associated factor 5 antibody
    • TRAF 5 antibody
    • Traf5 antibody
    • TRAF5_HUMAN antibody
    see all

Images

  • Anti-TRAF5 antibody (ab137763) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 64 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol fixed HeLa labeling TRAF5 with ab137763 at a 1/200 dilution.
    The lower image was costained with Hoechst 33342.

References

This product has been referenced in:

  • Cai Y & Li Y Upregulation of miR-29b-3p protects cardiomyocytes from hypoxia-induced apoptosis by targeting TRAF5. Cell Mol Biol Lett 24:27 (2019). Read more (PubMed: 31011336) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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