Anti-TRAF6 antibody (ab137452)

Rabbit polyclonal TRAF6 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Human. Cited in 2 publication(s). Immunogen corresponding to recombinant fragment.

Overview

  • Product name

  • Description

    Rabbit polyclonal to TRAF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 144-408 of Human TRAF6 (UniProt ID: Q9Y4K3).

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab137452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Post-translational
    modifications

    Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
    Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase TRAF6 antibody
    • Interleukin 1 signal transducer antibody
    • Interleukin-1 signal transducer antibody
    • MGC 3310 antibody
    • MGC:3310 antibody
    • MGC3310 antibody
    • OTTHUMP00000232772 antibody
    • OTTHUMP00000232773 antibody
    • RING finger protein 85 antibody
    • RNF 85 antibody
    • RNF85 antibody
    • TNF receptor associated factor 6 antibody
    • TNF receptor-associated factor 6 antibody
    • TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody
    • TRAF 6 antibody
    • Traf6 antibody
    • TRAF6_HUMAN antibody
    see all

Images

  • All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution

    Lane 1 : A549 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • All lanes : Anti-TRAF6 antibody (ab137452) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : BCL-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 60 kDa

  • Immunofluorescence analysis of methanol-fixed HeLa cells, labelling TRAF6 using ab137452 at 1/200 dilution. Right panel costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded 59T xenograft tissue, labelling TRAF6 using ab137452 at 1/500 dilution.

References

This product has been referenced in:

  • Liu L & Steinle JJ Loss of TLR4 in mouse Müller cells inhibits both MyD88-dependent and -independent signaling. PLoS One 12:e0190253 (2017). WB ; Mouse . Read more (PubMed: 29287085) »
  • Berger EA  et al. ß-Adrenergic receptor agonist, compound 49b, inhibits TLR4 signaling pathway in diabetic retina. Immunol Cell Biol 94:656-61 (2016). WB ; Rat . Read more (PubMed: 26888251) »
See all 2 Publications for this product

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