Anti-TRAF6 antibody (ab62488)
Key features and details
- Rabbit polyclonal to TRAF6
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRAF6 antibody
See all TRAF6 primary antibodies -
Description
Rabbit polyclonal to TRAF6 -
Host species
Rabbit -
Specificity
This antibody reacts with TRAF6 -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanICC/IF HumanWB Human
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Immunogen
A 14 amino acid synthetic peptide from near the C terminus of human TRAF6
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Positive control
- Purchase matching WB positive control:Recombinant Human TRAF6 protein
- PC3 cell lysate and K562 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| ICC/IF |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
|
| ICC |
Use a concentration of 0.5 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
|
ICC
Use a concentration of 0.5 µg/ml. |
Target
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Function
E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
Post-translational
modificationsSumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. -
Cellular localization
Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7189 Human
- Omim: 602355 Human
- SwissProt: Q9Y4K3 Human
- Unigene: 444172 Human
- Unigene: 591983 Human
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Alternative names
- E3 ubiquitin-protein ligase TRAF6 antibody
- Interleukin 1 signal transducer antibody
- Interleukin-1 signal transducer antibody
see all
Images
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Western blot - Anti-TRAF6 antibody (ab62488)All lanes : Anti-TRAF6 antibody (ab62488) at 1 µg/ml
Lane 1 : PC3 cell lysates, with 1 µg blocking peptide
Lane 2 : PC3 cell lysates, without blocking peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-rabbit IgG
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunocytochemistry - Anti-TRAF6 antibody (ab62488)Immunocytochemistry of TRAF6 in K562 cells with ab62488 antibody at 0.5 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (ab62488)Immunofluorescence of TRAF6 in K562 cells using ab62488 at 20 ug/ml.
Protocols
Datasheets and documents
References (3)
ab62488 has been referenced in 3 publications.
- Kong L et al. MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). Med Sci Monit 24:3056-3067 (2018). PubMed: 29745371
- Wang XL et al. Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway. PLoS One 11:e0153919 (2016). ICC/IF ; Human . PubMed: 27120457
- Geetha T et al. Polyubiquitination of the neurotrophin receptor p75 directs neuronal cell survival. Biochem Biophys Res Commun 421:286-90 (2012). WB ; Mouse . PubMed: 22503986
