Anti-TRAF6 antibody (ab62488)
Key features and details
- Rabbit polyclonal to TRAF6
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRAF6 antibody
See all TRAF6 primary antibodies -
Description
Rabbit polyclonal to TRAF6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
A 14 amino acid synthetic peptide from near the C terminus of human TRAF6
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Positive control
- Purchase matching WB positive control:Recombinant Human TRAF6 protein
- PC3 cell lysate and K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab62488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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| ICC |
Use a concentration of 0.5 - 20 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
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ICC
Use a concentration of 0.5 - 20 µg/ml. |
Target
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Function
E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers. -
Domain
The coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains. -
Post-translational
modificationsSumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. -
Cellular localization
Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7189 Human
- Omim: 602355 Human
- SwissProt: Q9Y4K3 Human
- Unigene: 591983 Human
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Alternative names
- E3 ubiquitin-protein ligase TRAF6 antibody
- Interleukin 1 signal transducer antibody
- Interleukin-1 signal transducer antibody
see all
Images
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Western blot - Anti-TRAF6 antibody (ab62488)All lanes : Anti-TRAF6 antibody (ab62488) at 1 µg/ml
Lane 1 : PC3 cell lysates, with 1 µg blocking peptide
Lane 2 : PC3 cell lysates, without blocking peptide
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti-rabbit IgG
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunocytochemistry - Anti-TRAF6 antibody (ab62488)Immunocytochemistry of TRAF6 in K562 cells with ab62488 antibody at 0.5 µg/ml. -
Immunocytochemistry - Anti-TRAF6 antibody (ab62488)Immunofluorescence of TRAF6 in K562 cells using ab62488 at 20 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab62488 has been referenced in 4 publications.
- Liu CP et al. miR-146a reduces depressive behavior by inhibiting microglial activation. Mol Med Rep 23:N/A (2021). PubMed: 33880591
- Kong L et al. MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). Med Sci Monit 24:3056-3067 (2018). PubMed: 29745371
- Wang XL et al. Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway. PLoS One 11:e0153919 (2016). ICC/IF ; Human . PubMed: 27120457
- Geetha T et al. Polyubiquitination of the neurotrophin receptor p75 directs neuronal cell survival. Biochem Biophys Res Commun 421:286-90 (2012). WB ; Mouse . PubMed: 22503986
