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    traf6-antibody-ab62488.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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Anti-TRAF6 antibody (ab62488)

  • Datasheet
Submit a review Submit a question References (3)

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Western blot - Anti-TRAF6 antibody (ab62488)
  • Immunocytochemistry - Anti-TRAF6 antibody (ab62488)
  • Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (ab62488)

Key features and details

  • Rabbit polyclonal to TRAF6
  • Suitable for: ICC/IF, WB, ICC
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-TRAF6 antibody
    See all TRAF6 primary antibodies
  • Description

    Rabbit polyclonal to TRAF6
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with TRAF6
  • Tested Applications & Species

    Application Species
    ICC
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    A 14 amino acid synthetic peptide from near the C terminus of human TRAF6

  • Positive control

    • Purchase matching WB positive control:Recombinant Human TRAF6 protein
    • PC3 cell lysate and K562 cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • TRAF
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • TRAF
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human TRAF6 protein (ab132023)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab62488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
ICC/IF
Human
WB
Human
Application Abreviews Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC
Use a concentration of 0.5 µg/ml.
Notes
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC
Use a concentration of 0.5 µg/ml.

Target

  • Function

    E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Post-translational
    modifications

    Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
    Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
  • Target information above from: UniProt accession Q9Y4K3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7189 Human
    • Omim: 602355 Human
    • SwissProt: Q9Y4K3 Human
    • Unigene: 444172 Human
    • Unigene: 591983 Human
    • Alternative names

      • E3 ubiquitin-protein ligase TRAF6 antibody
      • Interleukin 1 signal transducer antibody
      • Interleukin-1 signal transducer antibody
      • MGC 3310 antibody
      • MGC:3310 antibody
      • MGC3310 antibody
      • OTTHUMP00000232772 antibody
      • OTTHUMP00000232773 antibody
      • RING finger protein 85 antibody
      • RNF 85 antibody
      • RNF85 antibody
      • TNF receptor associated factor 6 antibody
      • TNF receptor-associated factor 6 antibody
      • TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody
      • TRAF 6 antibody
      • Traf6 antibody
      • TRAF6_HUMAN antibody
      see all

    Images

    • Western blot - Anti-TRAF6 antibody (ab62488)
      Western blot - Anti-TRAF6 antibody (ab62488)
      All lanes : Anti-TRAF6 antibody (ab62488) at 1 µg/ml

      Lane 1 : PC3 cell lysates, with 1 µg blocking peptide
      Lane 2 : PC3 cell lysates, without blocking peptide

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Anti-rabbit IgG

      Predicted band size: 63 kDa
      Observed band size: 63 kDa

    • Immunocytochemistry - Anti-TRAF6 antibody (ab62488)
      Immunocytochemistry - Anti-TRAF6 antibody (ab62488)
      Immunocytochemistry of TRAF6 in K562 cells with ab62488 antibody at 0.5 µg/ml.
    • Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (ab62488)
      Immunocytochemistry/ Immunofluorescence - Anti-TRAF6 antibody (ab62488)
      Immunofluorescence of TRAF6 in K562 cells using ab62488 at 20 ug/ml.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab62488? Please let us know so that we can cite the reference in this datasheet.

    ab62488 has been referenced in 3 publications.

    • Kong L  et al. MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). Med Sci Monit 24:3056-3067 (2018). PubMed: 29745371
    • Wang XL  et al. Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway. PLoS One 11:e0153919 (2016). ICC/IF ; Human . PubMed: 27120457
    • Geetha T  et al. Polyubiquitination of the neurotrophin receptor p75 directs neuronal cell survival. Biochem Biophys Res Commun 421:286-90 (2012). WB ; Mouse . PubMed: 22503986

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