Product nameAnti-TRAF6 antibody
See all TRAF6 primary antibodies
DescriptionRabbit polyclonal to TRAF6
SpecificityThis antibody reacts with TRAF6
Tested applicationsSuitable for: ICC/IF, WB, ICCmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid synthetic peptide from near the C terminus of human TRAF6
- Purchase matching WB positive control:Recombinant Human TRAF6 protein
- PC3 cell lysate and K562 cells
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityIon Exchange Chromatography
Our Abpromise guarantee covers the use of ab62488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|ICC||Use a concentration of 0.5 µg/ml.|
FunctionE3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the TNF receptor-associated factor family. A subfamily.
Contains 1 MATH domain.
Contains 1 RING-type zinc finger.
Contains 2 TRAF-type zinc fingers.
DomainThe coiled coil domain mediates homo- and hetero-oligomerization.
The MATH/TRAF domain binds to receptor cytoplasmic domains.
modificationsSumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
Cellular localizationCytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
- Information by UniProt
- E3 ubiquitin-protein ligase TRAF6 antibody
- Interleukin 1 signal transducer antibody
- Interleukin-1 signal transducer antibody
All lanes : Anti-TRAF6 antibody (ab62488) at 1 µg/ml
Lane 1 : PC3 cell lysates, with 1 µg blocking peptide
Lane 2 : PC3 cell lysates, without blocking peptide
Lysates/proteins at 15 µg per lane.
All lanes : Anti-rabbit IgG
Predicted band size: 63 kDa
Observed band size: 63 kDa
Immunocytochemistry of TRAF6 in K562 cells with ab62488 antibody at 0.5 µg/ml.
Immunofluorescence of TRAF6 in K562 cells using ab62488 at 20 ug/ml.
This product has been referenced in:
- Kong L et al. MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). Med Sci Monit 24:3056-3067 (2018). Read more (PubMed: 29745371) »
- Wang XL et al. Rosuvastatin Attenuates CD40L-Induced Downregulation of Extracellular Matrix Production in Human Aortic Smooth Muscle Cells via TRAF6-JNK-NF-?B Pathway. PLoS One 11:e0153919 (2016). ICC/IF ; Human . Read more (PubMed: 27120457) »