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    traf6-antibody-ep591y-ab33915.pdf

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Cell Biology Apoptosis Receptors Associated Proteins
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-TRAF6 antibody [EP591Y] (ab33915)

  • Datasheet
  • SDS
Reviews (9)Q&A (7)References (93)

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Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
  • Anti-TRAF6 antibody [EP591Y] (ab33915)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP591Y] to TRAF6
  • Suitable for: Flow Cyt (Intra), WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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Overview

  • Product name

    Anti-TRAF6 antibody [EP591Y]
    See all TRAF6 primary antibodies
  • Description

    Rabbit monoclonal [EP591Y] to TRAF6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Zebrafish
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab183540)

  • Positive control

    • HeLa, Daudi, Jurkat and HAP1 whole cell lysate. Human colon adenocarcinoma. This antibody is unsuitable for detecting TRAF6 in tissue lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP591Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Receptors
    • Associated Proteins
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • NFkB Pathway
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Cancer
    • Growth factors
    • TNF
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Death receptors & ligands
    • TRAF
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Death receptors & ligands
    • TRAF
    • Cancer
    • Cell Death
    • Apoptosis
    • Receptors
    • Associated Proteins

Associated products

  • Alternative Versions

    • PE Anti-TRAF6 antibody [EP591Y] (ab210412)
    • Alexa Fluor® 488 Anti-TRAF6 antibody [EP591Y] (ab214638)
    • Alexa Fluor® 647 Anti-TRAF6 antibody [EP591Y] (ab214941)
    • Anti-TRAF6 antibody [EP591Y] - BSA and Azide free (ab218575)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Immunizing Peptide (Blocking)

    • TRAF6 peptide (ab183540)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human TRAF6 knockout HeLa cell line (ab266009)
  • KO cell lysates

    • Human TRAF6 knockout HeLa cell lysate (ab257760)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human TRAF6 protein (ab132023)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab33915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (8)
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.Can be blocked with TRAF6 peptide (ab183540).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.Can be blocked with TRAF6 peptide (ab183540).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for IP.

Target

  • Function

    E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • Domain

    The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • Post-translational
    modifications

    Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
    Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
  • Cellular localization

    Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
  • Target information above from: UniProt accession Q9Y4K3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7189 Human
    • Entrez Gene: 22034 Mouse
    • Entrez Gene: 311245 Rat
    • Entrez Gene: 554561 Zebrafish
    • Omim: 602355 Human
    • SwissProt: Q9Y4K3 Human
    • SwissProt: P70196 Mouse
    • SwissProt: B5DF45 Rat
    • SwissProt: Q6IWL4 Zebrafish
    • Unigene: 591983 Human
    • Unigene: 292729 Mouse
    • Unigene: 220435 Rat
    • Unigene: 74618 Zebrafish
    see all
  • Alternative names

    • E3 ubiquitin-protein ligase TRAF6 antibody
    • Interleukin 1 signal transducer antibody
    • Interleukin-1 signal transducer antibody
    • MGC 3310 antibody
    • MGC:3310 antibody
    • MGC3310 antibody
    • OTTHUMP00000232772 antibody
    • OTTHUMP00000232773 antibody
    • RING finger protein 85 antibody
    • RNF 85 antibody
    • RNF85 antibody
    • TNF receptor associated factor 6 antibody
    • TNF receptor-associated factor 6 antibody
    • TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody
    • TRAF 6 antibody
    • Traf6 antibody
    • TRAF6_HUMAN antibody
    see all

Images

  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    All lanes : Anti-TRAF6 antibody [EP591Y] (ab33915) at 1/2000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : TRAF6 knockout HeLa cell lysate
    Lane 3 : HAP1 cell lysate
    Lane 4 : Daudi cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?



    Lanes 1- 4: Merged signal (red and green). Green - ab33915 observed at 65 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab33915 was shown to react with TRAF6 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab266009 (knockout cell lysate ab257760) was used. Wild-type HeLa and TRAF6 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab33915 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-TRAF6 knockout cells (red line) stained with ab33915. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab33915, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) preadsorbed (ab150081) at 1/2000 dilution for 30 min at 22°C. A rabbit IgG isotype control antibody (ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-TRAF6 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF6 antibody [EP591Y] (ab33915)

    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using anti-TRAF6 (ab33915)

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)Image courtesy of an anonymous Abreview.

    ab33915 staining TRAF6 in Human platelet cells by intracellular flow cytometry.Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/200 dilution and incubated for 16 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution. 

    P : permeabilized US : Unstained (Red Peak) IGG RB : IgG Rabbit (Blue Peak) TRAF6 Ab (Green Peak)

     

    See Abreview

  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
    Lane 2: TRAF6 knockout  HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab33915 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Ab33915 was shown to specifically react with TRAF6 in wild-type cells as signal was lost in TRAF6 knockout HAP1 cells. Wild-type and TRAF6 knockout samples were subjected to SDS-PAGE.  Ab33915 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging

  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Anti-TRAF6 antibody [EP591Y] (ab33915) at 1/10000 dilution + Jurkat cell lysate

    Predicted band size: 58 kDa

  • Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Flow Cytometry (Intracellular) - Anti-TRAF6 antibody [EP591Y] (ab33915)

    Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling TRAF6 with purified ab33915 at 1/240 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

     

     

  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    All lanes : Anti-TRAF6 antibody [EP591Y] (ab33915) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Human skeletal muscle lysates
    Lane 4 : Mouse skeletal muscle lysates
    Lane 5 : Rat skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 58 kDa



    This antibody is unsuitable for detecting tissue lysates.

  • Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    Western blot - Anti-TRAF6 antibody [EP591Y] (ab33915)
    All lanes : Anti-TRAF6 antibody [EP591Y] (ab33915) at 1/1000 dilution

    Lane 1 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 58 kDa

  • Anti-TRAF6 antibody [EP591Y] (ab33915)
    Anti-TRAF6 antibody [EP591Y] (ab33915)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (93)

Publishing research using ab33915? Please let us know so that we can cite the reference in this datasheet.

ab33915 has been referenced in 93 publications.

  • Xu X  et al. MicroRNA-139-5p inhibits inflammatory and oxidative stress responses of Salmonella-infected macrophages through modulating TRAF6. Pathog Dis 79:N/A (2021). PubMed: 33822016
  • Huang C  et al. Hepatocyte growth factor overexpression promotes osteoclastogenesis and exacerbates bone loss in CIA mice. J Orthop Translat 27:9-16 (2021). PubMed: 33344167
  • Zhang S  et al. Activation of Toll-Like Receptor 7 Signaling Pathway in Primary Sjögren's Syndrome-Associated Thrombocytopenia. Front Immunol 12:637659 (2021). PubMed: 33767707
  • Wang WX  et al. Temporal changes in inflammatory mitochondria-enriched microRNAs following traumatic brain injury and effects of miR-146a nanoparticle delivery. Neural Regen Res 16:514-522 (2021). PubMed: 32985480
  • Fan X & Wang Y Circular RNA circSPATA6 Inhibits the Progression of Oral Squamous Cell Carcinoma Cells by Regulating TRAF6 via miR-182. Cancer Manag Res 13:1817-1829 (2021). PubMed: 33654430
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 16 Abreviews or Q&A

Western blot abreview for Anti-TRAF6 antibody [EP591Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 17 2014

Western blot abreview for Anti-TRAF6 antibody [EP591Y]

Below Average
Abreviews
Abreviews
Application
Western blot
Loading amount
7.5 µg
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Sample
Zebrafish Tissue lysate - whole (embryos 5-7 days post fertilization)
Specification
embryos 5-7 days post fertilization
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Rub�n Valued Customer

Verified customer

Submitted Oct 22 2013

Western blot abreview for Anti-TRAF6 antibody [EP591Y]

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa Cells)
Loading amount
25 µg
Specification
HeLa Cells
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 01 2012

Western blot abreview for Anti-TRAF6 antibody [EP591Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Mahesh Shivananjappa

Verified customer

Submitted Nov 23 2011

Flow Cytometry abreview for Anti-TRAF6 antibody [EP591Y]

Average
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 21 2010

Western blot abreview for Anti-TRAF6 antibody [EP591Y]

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW264.7)
Loading amount
50000 cells
Specification
RAW264.7
Treatment
100 ng/ml recombinant mouse RANKL
Gel Running Conditions
Reduced Denaturing (NuPage 10% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 01 2010

Question

Is this antibody truly a monoclonal? (I have never heard of a rabbitmonoclonal.)
https://www.abcam.com/TRAF6-antibody-EP591Y-ab33915.html

Read More

Abcam community

Verified customer

Asked on Mar 26 2012

Answer

Thank you for contacting Abcam and for your interest in ab33915.


I can confirm for you that this is indeed a rabbit monoclonal antibody. Rabbit antibodies are generally high affinity and are an ideal species to choose for making antibodies against mouse and rat proteins as many antibodies made in mice or rats will not react against mouse or rat proteins. From our experiences the rabbit immune system appears to be capable of generating affinities against small epitopes which are not recognized by the mouse.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions or concerns.

Read More

Abcam Scientific Support

Answered on Mar 26 2012

Question

Can you recommend EasyLink conjugation kits for bothe IRAK1 and TRAF6 kits, please.

Read More

Abcam community

Verified customer

Asked on Feb 23 2012

Answer

Thank you for your reply.

Our IRAK antibody ab238 will be compatible with any of our EasyLink conjugation kits. The full list of available sizes and fluorophores can be found at https://www.abcam.com/EasyLink, but for example you could use the FITC conjugation kit ab102885.

The TRAF6 antibody ab33915 is a rabbit monoclonal. Because the binding affinity of rabbit IgG is much, much higher than that of mouse IgG, a rabbit monoclonal antibody will be suitable for use at a far lower concentration. For that reason, ab33915 is sold at a very low concentration. The EasyLink kits can only be used with greater than 10ug of antibody per conjugation, in a volume of 4-10ul. That being said, even with the low concentration, we will guarantee this antibody to work at a dilution of 1:50 for Flow Cytometry.

I hope this helps, please let me know if you need any other information.

Read More

Abcam Scientific Support

Answered on Feb 23 2012

Question

I would like to know whether there are ELISA kits available for measuring TRAF6, IRAK1, STAT1 and IRF5 in serum?

Read More

Abcam community

Verified customer

Asked on Feb 14 2012

Answer

Thank you for contacting us.

We do not currently have any ELISA kits to TRAF6, IRAK1, or IRF5. We currently have three ELISA kits for STAT1 phosphorylations, but none to total STAT1:

ab119649: PhosphoTracer STAT1 (pY701) ELISA Kit

ab119671: PhosphoTracer STAT1 (pT701) + total GAPDH ELISA Kit

ab119678: PhosphoTracer STAT1 (pY701) + STAT3 (pY705) + STAT5 (pY694) ELISA Kit

We have several antibodies to IRAK (ab47843 and ab5522), STAT1 (ab118848 and ab64913), and IRF5 (ab33478 and ab49056)that would be suitable for use in ELISA, but none of our TRAF6 antibodies have been validated for this purpose. If you would like to test one of our antibodies in an untested species or application, I would be happy to send you more information about our testing discount program.

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Abcam Scientific Support

Answered on Feb 14 2012

Question

I would like to know whether you have any flow cytometry antibodies for TRAF6 and IRAK1? Or for TRAF6 and IRAK1 protein studies in whole or peripheral blood?

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Abcam community

Verified customer

Asked on Feb 13 2012

Answer

Thank you for contacting us. We currently have one antibody for each of these targets that has been validated for use in Flow Cytometry:

ab238 rabbit polyclonal to IRAK; tested for use in WB, IP, IHC-P, Flow Cyt, ICC/IF; reacts with human, mouse, and rat.

ab33915 rabbit monoclonal to TRAF6; tested for use in WB, IP, Flow Cyt, IHC-P; reacts with human, mouse, and rat.

Both of these antibodies are unconjugated, but you could use on of our EasyLink conjugation kits to directly conjugate ab238. If you are looking for an isotype control, you may be interested in ab27478.

https://www.abcam.com/EasyLink

The rabbit monoclonal ab33915 typically has a very low concentration because rabbit monoclonals have a much higher binding affinity than mouse monoclonals, but the hybridomas produce less antibody. For this reason, the concentration will be too low for use with our EasyLink conjugation kits, but it can be used with an anti-rabbit IgG secondary antibody.

I hope this helps, please let me know if you need any additional information.

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Abcam Scientific Support

Answered on Feb 13 2012

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