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Can you recommend EasyLink conjugation kits for bothe IRAK1 and TRAF6 kits, please.
Asked on Feb 23 2012
Thank you for your reply.
Our IRAK antibody ab238 will be compatible with any of our EasyLink conjugation kits. The full list of available sizes and fluorophores can be found at https://www.abcam.com/EasyLink, but for example you could use the FITC conjugation kit ab102885.
The TRAF6 antibody ab33915 is a rabbit monoclonal. Because the binding affinity of rabbit IgG is much, much higher than that of mouse IgG, a rabbit monoclonal antibody will be suitable for use at a far lower concentration. For that reason, ab33915 is sold at a very low concentration. The EasyLink kits can only be used with greater than 10ug of antibody per conjugation, in a volume of 4-10ul. That being said, even with the low concentration, we will guarantee this antibody to work at a dilution of 1:50 for Flow Cytometry.
I hope this helps, please let me know if you need any other information.
Answered on Feb 23 2012