Key features and details
- Rabbit polyclonal to TRAF6BP/TAX1BP1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRAF6BP/TAX1BP1 antibody
See all TRAF6BP/TAX1BP1 primary antibodies
DescriptionRabbit polyclonal to TRAF6BP/TAX1BP1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Synthetic peptide within Human TRAF6BP/TAX1BP1 aa 739-789. The exact sequence is proprietary. NP_006015.4
Database link: Q86VP1
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
This product was previously labelled as TRAF6BP
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245636 was affinity purified using an epitope specific to TRAF6BP/TAX1BP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 91 kDa.|
FunctionInhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. Degraded by caspase-3-like family proteins upon TNF-induced apoptosis. May also play a role in the pro-inflammatory cytokine IL-1 signaling cascade.
Tissue specificityExpressed in all tissues tested.
- Information by UniProt
- 1200003J11Rik antibody
- 1700069J21Rik antibody
- Aa1076 antibody
All lanes : Anti-TRAF6BP/TAX1BP1 antibody (ab245636) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30 seconds
TRAF6BP/TAX1BP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245636 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245636 at 1 µg/ml.
Lane 1: ab245636 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245636 has been referenced in 1 publication.
- Hu S et al. Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering. Nat Commun 10:3459 (2019). PubMed: 31371777