Recombinant
RabMAb

Recombinant Anti-TRAFD1 antibody [EPR12322] (ab174853)

Overview

  • Product name

    Anti-TRAFD1 antibody [EPR12322]
    See all TRAFD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12322] to TRAFD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human TRAFD1. The exact sequence is proprietary.
    Database link: O14545

  • Positive control

    • Human thymus; Human skeletal muscle; Human fetal brain; Human lymph node and Human spleen tissue lysates; Human brain tissue; Human cervix carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12322
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3.
    • Sequence similarities

      Contains 1 TRAF-type zinc finger.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLN29 antibody
      • FLN29 gene product antibody
      • Protein FLN29 antibody
      • TRAD1_HUMAN antibody
      • TRAF type zinc finger domain containing 1 antibody
      • TRAF type zinc finger domain containing protein 1 antibody
      • TRAF-type zinc finger domain-containing protein 1 antibody
      • TRAFD 1 antibody
      • TRAFD1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling TRAFD1 using ab174853 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TRAFD1 using ab174853 at 1/50 dilution.

    • All lanes : Anti-TRAFD1 antibody [EPR12322] (ab174853) at 1/1000 dilution

      Lane 1 : Human fetal thymus lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : Human fetal brain lysate
      Lane 4 : Human lymph node lysate
      Lane 5 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 65 kDa

    References

    ab174853 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174853.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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