• Product name
    Anti-TRAIL antibody [2E5]
    See all TRAIL primary antibodies
  • Description
    Mouse monoclonal [2E5] to TRAIL
  • Host species
  • Specificity
    This antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)). Purified from TCS
  • Tested applications
    Suitable for: Functional Studies, Flow Cyt, Neutralisingmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.



Our Abpromise guarantee covers the use of ab2219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.
Flow Cyt Use a concentration of 1 - 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


Neutralising Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.


  • Function
    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • Tissue specificity
    Widespread; most predominant in spleen, lung and prostate.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo 2 ligand antibody
    • APO 2L antibody
    • Apo-2 ligand antibody
    • Apo-2L antibody
    • APO2L antibody
    • CD253 antibody
    • CD253 antigen antibody
    • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
    • Protein TRAIL antibody
    • TL2 antibody
    • TNF Related Apoptosis Inducing Ligand antibody
    • TNF related apoptosis inducing ligand TRAIL antibody
    • TNF-related apoptosis-inducing ligand antibody
    • TNF10_HUMAN antibody
    • TNFSF10 antibody
    • TRAIL antibody
    • Tumor necrosis factor (ligand) family member 10 antibody
    • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
    • Tumor necrosis factor ligand superfamily member 10 antibody
    see all


  • Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
    The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
    A - medium
    B - recombinant TRAIL
    C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
    D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
    E - recombinant TRAIL + Isotype mouse IgG1 control


This product has been referenced in:
  • Tatzel K  et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . Read more (PubMed: 26935795) »
  • Yunker PJ  et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). Read more (PubMed: 26721399) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


This antibody was raised against soluble recombinant TRAIL /APO-2L (extracellular domain). It completely neutralizes apoptosis in cells induced by TRAIL (e.g. by soluble recombinant human TRAIL) that is why datasheet states functional application.

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Thank you for contacting us.
I have attached the data for the Functional Studies done with ab2219. We will add these to the datasheet as well very soon.
Also, I wanted to send you the information about our 3 for 2 promotion for all proteins that is going on until the end of the month.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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