Product nameAnti-TRAIL antibody [2E5]
See all TRAIL primary antibodies
DescriptionMouse monoclonal [2E5] to TRAIL
SpecificityThis antibody is specific for TRAIL, Tumour necrosis factor (TNF) related apoptosis inducing ligand (TRAIL/ APO2-L)). Purified from TCS
Tested applicationsSuitable for: Functional Studies, Flow Cyt, Neutralisingmore details
Species reactivityReacts with: Human
Does not react with: Mouse
Recombinant soluble fragment, corresponding to amino acids 95-281 of Human TRAIL.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: None
Constituents: PBS, pH 7.4
Concentration information loading...
Purity>95% by SDS-PAGE
Light chain typeunknown
Our Abpromise guarantee covers the use of ab2219 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.|
|Flow Cyt||Use a concentration of 1 - 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Neutralising||Use at an assay dependent concentration. The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays.|
FunctionCytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Tissue specificityWidespread; most predominant in spleen, lung and prostate.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5).
The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL.
A - medium
B - recombinant TRAIL
C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 µg/ml)
D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 µg/ml)
E - recombinant TRAIL + Isotype mouse IgG1 control
This product has been referenced in:
- Tatzel K et al. Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Sci Rep 6:22661 (2016). Flow Cyt . Read more (PubMed: 26935795) »
- Yunker PJ et al. One-pot system for synthesis, assembly, and display of functional single-span membrane proteins on oil-water interfaces. Proc Natl Acad Sci U S A 113:608-13 (2016). Read more (PubMed: 26721399) »