Overview

  • Product name
    Anti-TRAIL antibody [75411.11]
    See all TRAIL primary antibodies
  • Description
    Mouse monoclonal [75411.11] to TRAIL
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, Inhibition Assay, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein (Human).

  • General notes

    To measure the ability of this antibody to neutralize the biological activity of TRAIL, cross-linked recombinant human TRAIL is incubated with various concentrations of the antibody for 1 hour at 37 oC in a 96 well plate. Following this preincubation period, mouse L929 cells are added. The assay mixture in a total volume of 150 μL per well, containing antibody at concentrations of 0.01-100 ng/mL, cross-linked recombinant human TRAIL at 12 ng/mL, and actinomycin D at 1 μg/mL, are incubated at 37 oC for 24 hours in a humidified CO2 incubator. Following incubation, the cells are fixed with 5% formaldehyde and then stained with a crystal violet solution containing 100 μL of 33% acetic acid. Absorbance at 540 nm is read on a microplate reader.

Properties

Applications

Our Abpromise guarantee covers the use of ab10516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 8 - 25 µg/ml.
Inhibition Assay Use a concentration of 0.02 - 0.08 µg/ml.

The Neutralization Dose50 (ND50) for this antibody is defined as that concentration required to yield one-half maximal inhibition of the TRAIL-mediated activity on a responsive cell line, when TRAIL is present at a concentration high enough to elicit a maximum response. Monoclonal anti-TRAIL (APO-2L) has the ability to neutralize the biological activity of recombinant human TRAIL. The ND50 for this effect is approximately 2-8 ng/ml in the presence of 12 ng/ml of cross-linked recombinant human TRAIL, using mouse L929 cells.

The exact concentration of antibody required to neutralize TRAIL is dependent on the cytokine concentration, cell type, growth conditions and the type of activity.

ELISA Use a concentration of 0.5 - 1 µg/ml.

Concentration determined using 25ng/well recombinant human TRAIL.

WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 33.7 kDa). PubMed: 21419121

Determined using human TRAIL at approximately 50 ng/lane under non-reducing and reducing conditions. 

Target

  • Function
    Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
  • Tissue specificity
    Widespread; most predominant in spleen, lung and prostate.
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo 2 ligand antibody
    • APO 2L antibody
    • Apo-2 ligand antibody
    • Apo-2L antibody
    • APO2L antibody
    • CD253 antibody
    • CD253 antigen antibody
    • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
    • Protein TRAIL antibody
    • TL2 antibody
    • TNF Related Apoptosis Inducing Ligand antibody
    • TNF related apoptosis inducing ligand TRAIL antibody
    • TNF-related apoptosis-inducing ligand antibody
    • TNF10_HUMAN antibody
    • TNFSF10 antibody
    • TRAIL antibody
    • Tumor necrosis factor (ligand) family member 10 antibody
    • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
    • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
    • Tumor necrosis factor ligand superfamily member 10 antibody
    see all

References

This product has been referenced in:
  • Wang MS  et al. Iron oxide magnetic nanoparticles combined with actein suppress non-small-cell lung cancer growth in a p53-dependent manner. Int J Nanomedicine 12:7627-7651 (2017). WB ; Human . Read more (PubMed: 29089760) »
  • Sambandam Y  et al. Vitamin D Modulation of TRAIL Expression in Human Milk and Mammary Epithelial Cells. Sci Rep 7:4362 (2017). WB ; Human . Read more (PubMed: 28659589) »
See all 5 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Question
Answer

I can confirm that our products are sterile and ab10516 also contains sodium azide and thimerisol as preservatives to keep the antibody sterile after opening. The preservatives are in a low concentration and should therefore not interfere with experiments when the antibody is used diluted.

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Question
Answer

That is no problem. If you do have any further questions, please do not hesitate to contact us again.

Until then, I wish you all the best with your research.

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Answer

Thank you for your reply.

The BSA is added to the antibody buffer improve long term storage. If you are making up a diluted solution to use in your assays you should not need to add BSA as long as you are not intending to store the solution for a long time.

I would have a look at how other researchers have set up the experiment you are trying to perform previously. This can be found by researching the relevant literature in your research area. I would suggest diluting the antibody to the required level in PBSand/or themedia you have been using for your cell culture.

I hope this information has been of help. If youhave any further questions, please do not hesitate to ask.

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Answer

Thank you for contacting us and your interest in our products.

We have information that this antibody has inhibition activity from the following reference:

Roux S et al. CD4+CD25+ Tregs control the TRAIL-dependent cytotoxicity of tumor-infiltrating DCs in rodent models of colon cancer. J Clin Invest 118:3751-61 (2008).

Unfortunately no reference of which exact buffer was used. However, I would suggest dilution the antibody in PBS and/or media would be sufficient. In order to design your experiment I would advise having a look at what other researchers have done in order to see how you would like to set the experiment up to obtain the results that you are hoping to observe.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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Answer

Nous sommes désolés d'apprendre que l'anticorps ab10516 n'a pas fonctionné comme attendu. Je me permets de vous informer à nouveau que nos produits sont garantis pour fonctionner tels qu'ils sont décrits sur la fiche technique et que nous pouvons donc toujours résoudre le problème que vous avez rencontré en vous remboursant, vous faire un avoir, ou vous envoyer un anticorps de remplacement. En échange, nous serions ravis si vous pouviez nous communiquer quelques détails concernant votre protocole en remplissant le questionnaire ci-joint. Ces informations sont très importantes pour nous car elles nous permettent d'investiguer la source du problème rencontré avec cet anticorps et de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits.

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Answer

Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application or cross-reactivity with a species for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

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