Key features and details
- Rabbit polyclonal to TRAIL
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-TRAIL antibody
See all TRAIL primary antibodies
DescriptionRabbit polyclonal to TRAIL
Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide from internal of human CD253.
- Extracts from 293 cells and HUVEC cells, and A549 cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
|WB||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).|
FunctionCytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Tissue specificityWidespread; most predominant in spleen, lung and prostate.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
These images show A549 cells, stained with ab65121 at 1/500 - 1/1000 dilution; with and without the immunising peptide.
All lanes : Anti-TRAIL antibody (ab65121) at 1/500 dilution
Lane 1 : extracts from 293 cells
Lane 2 : extracts from Huvec cells
Lane 3 : extracts from Huvec cells with immunising peptide
Predicted band size: 32 kDa
Observed band size: 32 kDa
Additional bands at: ~48 kDa, ~60 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
ab65121 has been referenced in 1 publication.
- Ru Q et al. Preclinical study of rAAV2-sTRAIL: pharmaceutical efficacy, biodistribution and safety in animals. Cancer Gene Ther 24:251-258 (2017). PubMed: 28429751