Anti-TRAIL antibody (ab9959)
Key features and details
- Rabbit polyclonal to TRAIL
- Suitable for: Sandwich ELISA
- Isotype: IgG
Overview
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Product name
Anti-TRAIL antibody
See all TRAIL primary antibodies -
Description
Rabbit polyclonal to TRAIL -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragment
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Immunogen
Recombinant fragment corresponding to Human TRAIL aa 100 to the C-terminus. Highly pure (>98%). E.coli derived
Database link: P50591 -
General notes
Although some customers have been successful in IHC we no longer batch test in this application.
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| sELISA |
Recombinant fragment
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| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.1 µg/ml.
To detect TRAIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab12124 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant TRAIL. |
| Notes |
|---|
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Sandwich ELISA
Use a concentration of 0.1 µg/ml. To detect TRAIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab12124 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant TRAIL. |
Target
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Function
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis. -
Tissue specificity
Widespread; most predominant in spleen, lung and prostate. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
Images
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Sandwich ELISA - Anti-TRAIL antibody (ab9959)Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1µg/ml.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab9959 has been referenced in 4 publications.
- Lee GT et al. Dihydrotestosterone Increases Cytotoxic Activity of Macrophages on Prostate Cancer Cells via TRAIL. Endocrinology 160:2049-2060 (2019). PubMed: 31184711
- Li Q et al. Chemically modified liposomes carrying TRAIL target activated hepatic stellate cells and ameliorate hepatic fibrosis in vitro and in vivo. J Cell Mol Med N/A:N/A (2018). PubMed: 30592139
- He X et al. An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes. Nucleic Acids Res 43:3712-25 (2015). IHC-P . PubMed: 25779046
- Graves JD et al. Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity. Cancer Cell 26:177-89 (2014). WB ; Human . PubMed: 25043603