Anti-TRAIL antibody (ab9959)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-TRAIL antibody
See all TRAIL primary antibodies -
DescriptionRabbit polyclonal to TRAIL
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Host speciesRabbit
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Tested applicationsSuitable for: ELISA, Neutralising, WB, IHC-P, ICC/IF, Sandwich ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human TRAIL aa 114-281. Highly pure (>98%). E.coli derived
Sequence:MRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSF LSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKY TSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNE HLIDMDHEASFFGAFLVG
Database link: P50591 -
Positive control
- Recombinant human TRAIL protein (ab9960) can be used as a positive control in WB. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDA-MB-231
Properties
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FormLyophilised:Reconstitute with 200µl of sterile water.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferPBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab9959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. To detect hTRAIL by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTRAIL. | |
| Neutralising | Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL (30 ng/ml), a concentration of 0.5 - 0.8 mg/ml of this antibody is required. | |
| WB | Use at an assay dependent concentration. To detect hTRAIL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| Sandwich ELISA | Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1µg/ml with ab12124 as Capture. |
Target
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FunctionCytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
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Tissue specificityWidespread; most predominant in spleen, lung and prostate.
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Sequence similaritiesBelongs to the tumor necrosis factor family.
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Cellular localizationMembrane.
- Information by UniProt
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Database links
- Entrez Gene: 8743 Human
- Omim: 603598 Human
- SwissProt: P50591 Human
- Unigene: 478275 Human
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Alternative names
- Apo 2 ligand antibody
- APO 2L antibody
- Apo-2 ligand antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab9959)ab9959 staining TRAIL in human metastatic carcinoma of lymph nodes from breast tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.
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Immunocytochemistry/ Immunofluorescence - Anti-TRAIL antibody (ab9959)ICC/IF image of ab9959 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9959 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAIL antibody (ab9959)ab9959 staining TRAIL in human metastatic carcinoma of lymph nodes from breast tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen. -
Sandwich ELISA - Anti-TRAIL antibody (ab9959)Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1µg/ml.
Datasheets and documents
Protocols
References
This product has been referenced in:
- He X et al. An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes. Nucleic Acids Res 43:3712-25 (2015). IHC-P . Read more (PubMed: 25779046) »
- Graves JD et al. Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity. Cancer Cell 26:177-89 (2014). WB ; Human . Read more (PubMed: 25043603) »
