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    trail-antibody-ab9959.pdf

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Cell Biology Apoptosis Extracellular Signals Death Ligands
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Anti-TRAIL antibody (ab9959)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

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Sandwich ELISA - Anti-TRAIL antibody (ab9959)

    Key features and details

    • Rabbit polyclonal to TRAIL
    • Suitable for: Sandwich ELISA
    • Isotype: IgG

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    Overview

    • Product name

      Anti-TRAIL antibody
      See all TRAIL primary antibodies
    • Description

      Rabbit polyclonal to TRAIL
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      sELISA
      Recombinant fragment
      See all applications and species data
    • Immunogen

      Recombinant fragment corresponding to Human TRAIL aa 100 to the C-terminus. Highly pure (>98%). E.coli derived
      Database link: P50591

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Although some customers have been successful in IHC we no longer batch test in this application.

    Properties

    • Form

      Lyophilized:Reconstitute with 200µl of sterile water.
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Death receptors & ligands
      • TRAIL
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Receptors
      • Death receptors & ligands
      • TRAIL

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • Recombinant human TRAIL protein (ab9960)
    • Recombinant Protein

      • Recombinant human TRAIL protein (ab9960)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9959 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    sELISA
    Recombinant fragment
    Application Abreviews Notes
    Sandwich ELISA
    Use a concentration of 0.1 µg/ml.

    To detect TRAIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab12124 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant TRAIL.

    Notes
    Sandwich ELISA
    Use a concentration of 0.1 µg/ml.

    To detect TRAIL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ab12124 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant TRAIL.

    Target

    • Function

      Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
    • Tissue specificity

      Widespread; most predominant in spleen, lung and prostate.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P50591 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • Apo 2 ligand antibody
      • APO 2L antibody
      • Apo-2 ligand antibody
      • Apo-2L antibody
      • APO2L antibody
      • CD253 antibody
      • CD253 antigen antibody
      • Chemokine tumor necrosis factor ligand superfamily member 10 antibody
      • Protein TRAIL antibody
      • TL2 antibody
      • TNF Related Apoptosis Inducing Ligand antibody
      • TNF related apoptosis inducing ligand TRAIL antibody
      • TNF-related apoptosis-inducing ligand antibody
      • TNF10_HUMAN antibody
      • TNFSF10 antibody
      • TRAIL antibody
      • Tumor necrosis factor (ligand) family member 10 antibody
      • Tumor Necrosis Factor (ligand) Superfamily Member 10 antibody
      • Tumor necrosis factor apoptosis inducing ligand splice variant delta antibody
      • Tumor necrosis factor ligand superfamily member 10 antibody
      see all

    Images

    • Sandwich ELISA - Anti-TRAIL antibody (ab9959)
      Sandwich ELISA - Anti-TRAIL antibody (ab9959)
      Standard curve for TRAIL (Analyte: ab78818); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [55B709.3] to TRAIL (ab12124) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to TRAIL (ab9959) at 0.1µg/ml.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab9959? Please let us know so that we can cite the reference in this datasheet.

    ab9959 has been referenced in 4 publications.

    • Lee GT  et al. Dihydrotestosterone Increases Cytotoxic Activity of Macrophages on Prostate Cancer Cells via TRAIL. Endocrinology 160:2049-2060 (2019). PubMed: 31184711
    • Li Q  et al. Chemically modified liposomes carrying TRAIL target activated hepatic stellate cells and ameliorate hepatic fibrosis in vitro and in vivo. J Cell Mol Med N/A:N/A (2018). PubMed: 30592139
    • He X  et al. An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes. Nucleic Acids Res 43:3712-25 (2015). IHC-P . PubMed: 25779046
    • Graves JD  et al. Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity. Cancer Cell 26:177-89 (2014). WB ; Human . PubMed: 25043603

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Imaging Mass Cytometry™ abreview for Anti-TRAIL antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Mass Cytometry
    Sample
    Human Tissue sections (Liver)
    Specification
    Liver
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 19 2020

    Question

    Dear Sir, As you mentioned in your data sheet for (ab9959) it can be used as Detector Antibody with mouse monoclonal [55B709.3] to TRAIL (ab12124)as Capture Antibody in a Sandwich ELISA to detect TRAIL protein (Human) (ab78818). 1. my question is that, if synthetic peptide, corresponding to amino acids 17-35 of Human TRAIL is used as immunogen for production of (ab12124), how it can be used for detection of (ab78818) which is a recombinant fragment corresponding to amino acids 114-281 of human TRAIL? 2. Is there any problem in using TRAIL antibody (ab9959) for detection of TRAIL recombinant protein(ab78818)? becuase full length highly pure (>98%) recombinant hTRAIL here is used as immunogen for its production? Thank you in advanced for you kind attention

    Read More

    Abcam community

    Verified customer

    Asked on Oct 05 2011

    Answer

    Thank you for your enquiry. I am pleased to help answer your questions. 1. I am sorry to confirm that ab12124 antibody would not be suitable for detection of the ab78818 protein. As you have noticed from the different amino acid sequences, they are not designed to be used together. 2. ab9959 is raised against full length protein. As a polyclonal antibody, it will be detecting epitopes along the entire length of the protein. However, we could not confirm or guarantee that it would detect the shorter length recombinant ab78818 as the epitopes will not have been mapped. i would suggest to use a full length recombinant protein. I am sorry we do not have full length protein in the catalogue at this time. I am sorry there are no suitable protein standards to suggest on this occasion for use with both these antibodies together. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Oct 05 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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