Overview

  • Product name
  • Description
    Goat polyclonal to TRAK1
  • Host species
    Goat
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide:

    GAKLSKQTSLR,

    corresponding to C terminal amino acids 943-953 of Human TRAK1.

  • Positive control
    • Human Heart Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab28751 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/64000.
WB Use a concentration of 0.3 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 106 kDa).Can be blocked with TRAK1 peptide (943-953) (ab45670).

Target

  • Relevance
    The specific function of TRAK1 remains unknown. It interacts with O-GlcNAc transferase, RHOT1/Miro-1 and RHOT2/Miro-2. It shows high expression in spinal cord and moderate expression in all other tissues and specific brain regions examined.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • 106 kDa O GlcNAc transferase interacting protein antibody
    • KIAA1042 antibody
    • OGT(O Glc NAc transferase) interacting protein 106 KDa antibody
    • OIP106 antibody
    • Trafficking kinesin binding protein 1 antibody
    • Trafficking protein kinesin binding 1 antibody
    • TRAK 1 antibody
    see all

Images

  • Anti-TRAK1 antibody (ab28751) at 0.3 µg/ml + human heart lysate (RIPA buffer, 30µg total protein per lane)

    Predicted band size: 106 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    Primary incubation was for 1 hour.
    Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Ogawa F  et al. DISC1 complexes with TRAK1 and Miro1 to modulate anterograde axonal mitochondrial trafficking. Hum Mol Genet N/A:N/A (2013). WB, ICC/IF ; Human . Read more (PubMed: 24092329) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain)
Loading amount
30 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Dr. zerong you

Verified customer

Submitted Feb 14 2012

Question
Answer

DISCOUNT CODE: XXXX Expiration date: I am very pleased to hear you would like to accept our offer and test ab28751 in mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (breast cancer cell lines)
Loading amount
40 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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