Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab28751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 106 kDa).Can be blocked with TRAK1 peptide (943-953) (ab45670).|
RelevanceThe specific function of TRAK1 remains unknown. It interacts with O-GlcNAc transferase, RHOT1/Miro-1 and RHOT2/Miro-2. It shows high expression in spinal cord and moderate expression in all other tissues and specific brain regions examined.
- 106 kDa O GlcNAc transferase interacting protein antibody
- KIAA1042 antibody
- OGT(O Glc NAc transferase) interacting protein 106 KDa antibody
Anti-TRAK1 antibody (ab28751) at 0.3 µg/ml + human heart lysate (RIPA buffer, 30µg total protein per lane)
Predicted band size: 106 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Primary incubation was for 1 hour.
Detected by western blot using chemiluminescence.