Key features and details
- Potent, selective KCa3.1 channel blocker
- CAS Number: 289905-88-0
- Purity: > 99%
- Soluble in ethanol to 10 mM and in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
Product nameTRAM 34, KCa3.1 channel blocker
DescriptionPotent, selective KCa3.1 channel blocker
Biological descriptionPotent, selective KCa3.1 channel blocker (IC50 = 18 nM). Atherosclerosis and immunosuppressive agent. Active in vitro and in vivo.
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in ethanol to 10 mM and in DMSO to 50 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab141885, TRAM 34, KCa3.1 channel blocker
lomone Labs TRAM 34 blocks KCa3.1 currents in stably transfected 293T cells.
Currents were elicited by 150 ms voltage ramp from -120mV to +60mV, applied every 10 sec using whole-cell voltage clamp technique (Vh -80mV). A. Superimposed traces of KCa3.1 currents (plotted against the ramp voltage) measured under control conditions and following 2 minutes perfusion of 50 nM, 250 nM, and 1 µM TRAM 34 (ab141885) as indicated. B. Time course of KCa3.1 current (at 0 mV) amplitude change as a result of the application of raising concentrations of TRAM 34 (perfusion duration indicated by the horizontal bar, concentration as indicated).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab141885 has been referenced in 2 publications.
- Pinto MC et al. Regulation of TMEM16A by CK2 and Its Role in Cellular Proliferation. Cells 9:N/A (2020). PubMed: 32380794
- Pousinha PA et al. The Amyloid Precursor Protein C-Terminal Domain Alters CA1 Neuron Firing, Modifying Hippocampus Oscillations and Impairing Spatial Memory Encoding. Cell Rep 29:317-331.e5 (2019). PubMed: 31597094